Molecular detection of rifampin and ofloxacin resistance for patients who experience relapse of multibacillary leprosy.

Abstract	Molecular detection of rifampin resistance (rpoB analysis) in Mycobacterium leprae was determined for 49 patients who experienced relapse of multibacillary leprosy and for 34 untreated patients. Molecular detection of ofloxacin resistance (gyrA analysis) was determined for the 12 patients who experienced relapse and who had received ofloxacin. Results of molecular tests were compared with the reference susceptibility test in the mouse footpad. Overall, the efficiency of molecular detection--that is, positive DNA amplification--was 95%, whereas that of the in vivo test was 55% (P<.001). Results of molecular detection and in vivo test were fully concordant when both were available--that is, for 35 rifampin--sensitive cases of leprosy (no rpoB mutation), 4 ofloxacin-sensitive cases (no gyrA mutation), 11 rifampin-resistant cases (rpoB missense mutations), and 1 ofloxacin-resistant case (gyrA mutation). rpoB and gyrA analysis appears to be an effective method for detection of rifampin and ofloxacin resistance in patients with leprosy.
Authors	Emmanuelle Cambau, Pascale Bonnafous, Evelyne Perani, Wladimir Sougakoff, Baohong Ji, Vincent Jarlier
Journal	Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (Clin Infect Dis) Vol. 34 Issue 1 Pg. 39-45 (Jan 01 2002) ISSN: 1537-6591 [Electronic] United States
PMID	11731943 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibiotics, Antitubercular DNA, Bacterial Plant Proteins RpoB protein, Oryza sativa Ofloxacin DNA-Directed RNA Polymerases DNA Gyrase Rifampin
Topics	Adult Aged Antibiotics, Antitubercular (pharmacology) DNA Gyrase (analysis, genetics) DNA, Bacterial (analysis) DNA-Directed RNA Polymerases Disease Models, Animal Drug Resistance, Bacterial (genetics) Humans Leprosy (microbiology) Microbial Sensitivity Tests Middle Aged Mutation Mycobacterium leprae (drug effects, genetics) Ofloxacin (pharmacology) Plant Proteins (analysis, genetics) Recurrence Rifampin (pharmacology)

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