Abstract |
Mitogen-activated protein kinase and Phosphatidylinositol-3 kinase/Akt-mediated signaling pathways play a major role in controlling cell proliferation, differentiation and cell death. Phosphorylation and dephosphorylation of their specific Thr/Tyr residues is critical in determining their activity. We determined the expression pattern and activity of MAP kinases and Akt in Primitive Neuroectodermal Tumors ( PNETs). The kinase activity of extracellular signal-regulated kinase (ERK) was higher in both primary tumors and cell lines, as evident from the increased phosphorylation of ERK1 and ERK2. We did not observe the activation of C-jun N-terminal kinase (JNK) or p38 MAPK The expression of Raf-1, a kinase acting upstream of ERK, was significantly increased in primary tumors compared to normal brain. The PI-3 kinase-activated phosphorylation of Akt was also higher in primary tumors. These results suggest that activation of the Raf-1/ERK module of the MAP kinase pathway play an important role in PNETs.
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Authors | C Damodar Reddy, S Marwaha, R Patti, M Raghunath, A C Duhaime, L Sutton, P C Phillips |
Journal | Anticancer research
(Anticancer Res)
2001 Jul-Aug
Vol. 21
Issue 4A
Pg. 2733-8
ISSN: 0250-7005 [Print] Greece |
PMID | 11724348
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Proto-Oncogene Proteins
- AKT1 protein, human
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins c-akt
- Proto-Oncogene Proteins c-raf
- Mitogen-Activated Protein Kinases
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Topics |
- Brain Neoplasms
(enzymology)
- Humans
- MAP Kinase Signaling System
(physiology)
- Mitogen-Activated Protein Kinases
(biosynthesis, metabolism)
- Neuroectodermal Tumors, Primitive
(enzymology)
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins
(biosynthesis, metabolism)
- Proto-Oncogene Proteins c-akt
- Proto-Oncogene Proteins c-raf
(biosynthesis, metabolism)
- Tumor Cells, Cultured
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