Abstract | BACKGROUND: METHODS AND RESULTS: Male and female wild-type (iNOS(+/+)) mice and mice with homozygous deletion of the iNOS gene (iNOS(-/-)) were studied intact (INT) or after ovariectomy (OVX) and implantation of E(2) or vehicle (V) pellets. Mice were randomized to 8 groups based on sex, iNOS status, OVX, and treatment with E(2) or V. Twenty-eight days after carotid artery ligation, mice were euthanized, and occluded vessels were evaluated for neointima formation by morphometric analysis. There was a marked sexual dimorphism in neointima formation in both the iNOS(+/+) mice and the iNOS(-/-) mice. iNOS(+/+) INT females had a >90% reduction in neointima formation compared with iNOS(+/+) males, and iNOS(-/-) INT females had a 65% reduction in neointima formation compared with iNOS(-/-) males. The sexually dimorphic response was attenuated by OVX and restored by E(2) replacement in both iNOS(+/+) and iNOS(-/-) mice. CONCLUSIONS: These results demonstrate that the vasoprotective effects of E(2) after ligation vascular injury are, at least in part, independent of iNOS expression.
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Authors | T Tolbert, J A Thompson, P Bouchard, S Oparil |
Journal | Circulation
(Circulation)
Vol. 104
Issue 22
Pg. 2740-5
(Nov 27 2001)
ISSN: 1524-4539 [Electronic] United States |
PMID | 11723029
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Estrogens
- Estradiol
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
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Topics |
- Animals
- Body Weight
(drug effects)
- Carotid Artery Injuries
(drug therapy, enzymology, pathology)
- Disease Models, Animal
- Estradiol
(blood, pharmacology)
- Estrogens
(blood, pharmacology)
- Female
- Ligation
- Male
- Mice
- Mice, Inbred Strains
- Mice, Knockout
- Nitric Oxide Synthase
(deficiency, genetics, metabolism)
- Nitric Oxide Synthase Type II
- Ovariectomy
- Sex Factors
- Tunica Intima
(drug effects, pathology)
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