T-cell receptor gamma chain alternate reading frame protein (TARP) expression in prostate cancer cells leads to an increased growth rate and induction of caveolins and amphiregulin.

Abstract	Previously, we showed that prostate and prostate cancer cells express a truncated T-cell receptor gamma chain mRNA that uses an alternative reading frame to produce a novel nuclear T-cell receptor gamma chain alternate reading frame protein (TARP). TARP is expressed in the androgen-sensitive LNCaP prostate cancer cell line but not in the androgen-independent PC3 prostate cancer cell line, indicating that TARP may play a role in prostate cancer progression. To elucidate the function of TARP, we generated a stable PC3 cell line that expresses TARP in a constitutive manner. Expression of TARP in PC3 cells resulted in a more rapid growth rate with a 5-h decrease in doubling time. cDNA microarray analysis of 6538 genes revealed that caveolin 1, caveolin 2, amphiregulin, and melanoma growth stimulatory activity alpha were significantly up-regulated, whereas IL-1beta was significantly down-regulated in PC3 cells expressing TARP. We also demonstrated that TARP expression is up-regulated by testosterone in LNCaP cells that express a functional androgen receptor. These results suggest that TARP has a role in regulating growth and gene expression in prostate cancer cells.
Authors	C D Wolfgang, M Essand, B Lee, I Pastan
Journal	Cancer research (Cancer Res) Vol. 61 Issue 22 Pg. 8122-6 (Nov 15 2001) ISSN: 0008-5472 [Print] United States
PMID	11719440 (Publication Type: Journal Article)
Chemical References	AREG protein, human Amphiregulin Androgens Caveolins EGF Family of Proteins Glycoproteins Growth Substances Intercellular Signaling Peptides and Proteins Nuclear Proteins TARP
Topics	Amphiregulin Androgens (physiology) Caveolins (biosynthesis, genetics) Cell Division (genetics) EGF Family of Proteins Gene Expression Regulation, Neoplastic (physiology) Glycoproteins (biosynthesis, genetics) Growth Substances (biosynthesis, genetics) Humans Intercellular Signaling Peptides and Proteins Male Neoplasms, Hormone-Dependent (genetics, metabolism, pathology) Nuclear Proteins (biosynthesis, genetics, physiology) Prostatic Neoplasms (genetics, metabolism, pathology) Tumor Cells, Cultured

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