Cellular accumulation and retention of the technetium-99m-labelled hypoxia markers BRU59-21 and butylene amine oxime.

Abstract

BRU59-21 and 99mTc-butylene amine oxime (BnAO, HL91) are being evaluated for imaging hypoxia in tumors. Both tracers: 1) rapidly reached a plateau in aerobic Chinese hamster ovary cells in vitro but continuously accumulated in hypoxic cells; 2) ceased to accumulate when hypoxic cells were exposed to air; 3) showed approximately 40% retention upon washing the cells; 4) showed selective hypoxic accumulation only at 37 degrees C; 5) accumulation could be modulated by addition of electron-affinic compounds; and 6) exhibited higher accumulation in cells which overexpress cytochrome P450 reductase. Both BRU59-21 and 99mTc-BnAO share properties making them suitable for hypoxia imaging.

• Authors: X Zhang, T Melo, A M Rauth, J R Ballinger
• Journal: Nuclear medicine and biology (Nucl Med Biol) Vol. 28 Issue 8 Pg. 949-57 (Nov 2001) ISSN: 0969-8051 [Print] United States
• PMID: 11711315 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BRU 59-21
• Imidazoles
• Organotechnetium Compounds
• Radiopharmaceuticals

Topics

• Animals
• CHO Cells
• Cricetinae
• Hypoxia (diagnostic imaging, metabolism)
• Imidazoles (metabolism)
• Organotechnetium Compounds (metabolism)
• Radionuclide Imaging
• Radiopharmaceuticals (metabolism)