Autologous stem cell transplantation for paediatric-onset polyarteritis nodosa: changes in autoimmune phenotype in the context of reduced diversity of the T- and B-cell repertoires, and evidence for reversion from the CD45RO(+) to RA(+) phenotype.
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| Abstract |
We have studied immune reconstitution in a patient with paediatric-onset polyarteritis nodosa treated with high-dose immunosuppressive agents followed by stem cell rescue. The patient developed several new autoimmune phenomena over the 18 months after immunosuppression and stem cell rescue. Flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR) heteroduplex and isotype-specific RT-PCR analysis of immunoglobulin expression showed that the T- and B-cell repertoires were highly restricted in the first few months after treatment. The dominant T-cell clones seen after reconstitution were persistently expanded, were different from those which could be demonstrated before autologous stem cell transplantation, and were in the CD8(+) population. Our data also show that 12 months after treatment these expanded T-cell clones were within the CD45RA(+) population, suggesting that reversion from the CD45RO(+) to the CD45RA(+) phenotype had occurred in vivo.
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| Authors | L R Wedderburn, R Jeffery, H White, A Patel, H Varsani, D Linch, K Murray, P Woo |
| Journal | Rheumatology (Oxford, England) (Rheumatology (Oxford)) Vol. 40 Issue 11 Pg. 1299-307 (Nov 2001) ISSN: 1462-0324 [Print] England |
| PMID | 11709615 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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