Abstract |
On the basis of our previous finding that humulone, a bitter acid from beer hop extract, was a potent inhibitor of bone resorption and inhibited the catalytic activity of cyclooxygenase-2 (COX-2) and more potently the transcription of the COX-2 gene, we examined the effect of humulone on angiogenesis, using chick embryo chorioallantoic membranes (CAMs) and vascular endothelial and tumor cells. Humulone significantly prevented in vivo angiogenesis in CAM in a dose-dependent manner with an ED(50) of 1.5 microg/CAM. Humulone also inhibited in vitro tube formation of vascular endothelial cells. Moreover, it suppressed the proliferation of endothelial cells and the production of vascular endothelial growth factor ( VEGF), an angiogenic growth factor, in endothelial and tumor cells. Thus, humulone is a potent angiogenic inhibitor, and may be a novel powerful tool for the therapy of various angiogenic diseases involving solid tumor growth and metastasis.
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Authors | M Shimamura, T Hazato, H Ashino, Y Yamamoto, E Iwasaki, H Tobe, K Yamamoto, S Yamamoto |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 289
Issue 1
Pg. 220-4
(Nov 23 2001)
ISSN: 0006-291X [Print] United States |
PMID | 11708802
(Publication Type: Journal Article)
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Copyright | Copyright 2001 Academic Press. |
Chemical References |
- Cyclohexenes
- Endothelial Growth Factors
- Isoenzymes
- Lymphokines
- Nitrobenzenes
- Sulfonamides
- Terpenes
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
- N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
- Cyclooxygenase 2
- Prostaglandin-Endoperoxide Synthases
- humulon
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Topics |
- Allantois
(blood supply, drug effects)
- Animals
- Beer
(analysis)
- Cell Division
(drug effects)
- Cells, Cultured
- Chick Embryo
- Chorion
(blood supply, drug effects)
- Cyclohexenes
- Cyclooxygenase 2
- Endothelial Growth Factors
(biosynthesis)
- Endothelium, Vascular
(cytology, drug effects, metabolism)
- Isoenzymes
(antagonists & inhibitors)
- Lymphokines
(biosynthesis)
- Mice
- Neovascularization, Pathologic
(drug therapy)
- Neovascularization, Physiologic
(drug effects)
- Nitrobenzenes
(pharmacology)
- Prostaglandin-Endoperoxide Synthases
- Rats
- Sulfonamides
(pharmacology)
- Terpenes
(isolation & purification, pharmacology)
- Tumor Cells, Cultured
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
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