Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins.

Abstract	A chimera of the two immunodominant African swine fever (ASF) virus proteins p54 and p30 was constructed by insertion of the gene CP204L into a Not I restriction site of E183L gene. The resulting chimeric protein p54/30, expressed by a recombinant baculovirus in insect cells and in Trichoplusia ni larvae, retained antigenic determinants present in both proteins and reacted in Western blot with a collection of sera from inapparent ASF virus carrier pigs. Remarkably, pigs immunized with the chimeric protein developed neutralizing antibodies and survived the challenge with a virulent African swine fever virus, presenting a reduction of about two logs in maximum viremia titers with respect to control pigs. In conclusion, this study revealed that the constructed chimeric protein may have utility as a serological diagnostic reagent and for further immunological studies that may provide new insights on mechanisms of protective immunity to ASFV.
Authors	M G Barderas, F Rodríguez, P Gómez-Puertas, M Avilés, F Beitia, C Alonso, J M Escribano
Journal	Archives of virology (Arch Virol) Vol. 146 Issue 9 Pg. 1681-91 ( 2001) ISSN: 0304-8608 [Print] Austria
PMID	11699955 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibodies, Viral Immunodominant Epitopes Phosphoproteins Recombinant Fusion Proteins Viral Proteins Viral Structural Proteins Viral Vaccines p54 protein, African swine fever virus P30 protein, African Swine Fever Virus
Topics	African Swine Fever (diagnosis, prevention & control, virology) African Swine Fever Virus (immunology) Animals Antibodies, Viral (blood) Baculoviridae (genetics, metabolism) Cells, Cultured Immunoblotting Immunodominant Epitopes (genetics, immunology, metabolism) Macrophages, Alveolar (virology) Moths (virology) Neutralization Tests Phosphoproteins (genetics, immunology, metabolism) Recombinant Fusion Proteins (genetics, immunology, metabolism) Spodoptera Swine Vaccination Viral Proteins (genetics, immunology, metabolism) Viral Structural Proteins (genetics, immunology, metabolism) Viral Vaccines (genetics, immunology)

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