Abstract |
A chimera of the two immunodominant African swine fever (ASF) virus proteins p54 and p30 was constructed by insertion of the gene CP204L into a Not I restriction site of E183L gene. The resulting chimeric protein p54/30, expressed by a recombinant baculovirus in insect cells and in Trichoplusia ni larvae, retained antigenic determinants present in both proteins and reacted in Western blot with a collection of sera from inapparent ASF virus carrier pigs. Remarkably, pigs immunized with the chimeric protein developed neutralizing antibodies and survived the challenge with a virulent African swine fever virus, presenting a reduction of about two logs in maximum viremia titers with respect to control pigs. In conclusion, this study revealed that the constructed chimeric protein may have utility as a serological diagnostic reagent and for further immunological studies that may provide new insights on mechanisms of protective immunity to ASFV.
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Authors | M G Barderas, F Rodríguez, P Gómez-Puertas, M Avilés, F Beitia, C Alonso, J M Escribano |
Journal | Archives of virology
(Arch Virol)
Vol. 146
Issue 9
Pg. 1681-91
( 2001)
ISSN: 0304-8608 [Print] Austria |
PMID | 11699955
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Immunodominant Epitopes
- Phosphoproteins
- Recombinant Fusion Proteins
- Viral Proteins
- Viral Structural Proteins
- Viral Vaccines
- p54 protein, African swine fever virus
- P30 protein, African Swine Fever Virus
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Topics |
- African Swine Fever
(diagnosis, prevention & control, virology)
- African Swine Fever Virus
(immunology)
- Animals
- Antibodies, Viral
(blood)
- Baculoviridae
(genetics, metabolism)
- Cells, Cultured
- Immunoblotting
- Immunodominant Epitopes
(genetics, immunology, metabolism)
- Macrophages, Alveolar
(virology)
- Moths
(virology)
- Neutralization Tests
- Phosphoproteins
(genetics, immunology, metabolism)
- Recombinant Fusion Proteins
(genetics, immunology, metabolism)
- Spodoptera
- Swine
- Vaccination
- Viral Proteins
(genetics, immunology, metabolism)
- Viral Structural Proteins
(genetics, immunology, metabolism)
- Viral Vaccines
(genetics, immunology)
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