Abstract |
In vitro excystation studies were carried out on the metacercariae cysts of Paragonimus heterotremus obtained from naturally infected crabs Potamon spp. The effects of elastase, trypsin, trypsin-dog bile, trypsin- bile salt, and dithiothreitol (DTT) were examined. The trypsin-dog bile medium stimulated maximum excystation. Of the media that contained 1 mM DTT, the optimum conditions for the excystation were shown to be pH 9, temperature of 39-40 C, and osmolarity of 250-350 mOsm. The DTT acceleration was antagonized by all of the following 6 protease inhibitors: leupeptin (0.5-4 microg/ml), L-trans-epoxysuccinyl leucylamido (4-guanidine) butane (1-8 microM), N-tosyl- L-phenylalanine chloromethyl ketone (0.1-0.4 mM), N alpha-p-tosyl- L-lysine chloromethyl ketone (25-200 microg/ml), iodoacetic acid (0.5-4 mM), and phenylmethylsulfonyl fluoride (1-4 mM). These results suggest that a number of extrinsic and intrinsic factors may modulate excystation.
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Authors | P M Intapan, W Maleewong |
Journal | The Journal of parasitology
(J Parasitol)
Vol. 87
Issue 5
Pg. 1184-6
(Oct 2001)
ISSN: 0022-3395 [Print] United States |
PMID | 11695393
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cysteine Proteinase Inhibitors
- Pancreatic Elastase
- Trypsin
- Sodium Cholate
- Dithiothreitol
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Topics |
- Animals
- Bile
- Brachyura
(parasitology)
- Cysteine Proteinase Inhibitors
(pharmacology)
- Dithiothreitol
(antagonists & inhibitors, pharmacology)
- Hydrogen-Ion Concentration
- Pancreatic Elastase
(pharmacology)
- Paragonimus
(drug effects, growth & development)
- Sodium Cholate
(pharmacology)
- Temperature
- Thailand
- Trypsin
(pharmacology)
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