Isolation and biological activity of frankiamide.

Abstract

An antibiotic produced by the symbiotic actinomycete Frankia strain AiPs1 was isolated from culture broth using optimized thin-layer chromatography and high-performance liquid chromatography (HPLC) methods. The novel compound that was isolated, dubbed frankiamide, displayed antimicrobial activity against all 14 Gram-positive bacterial strains and six pathogenic fungal strains tested. The pathogenic actinomycete Clavibacter michiganensis and the oomycete Phytophthora were especially susceptible. In addition to displaying antimicrobial activity, frankiamide also strongly inhibited 45Ca(2+) fluxes in clonal rat pituitary GH4C1 tumor cells and was comparable to a frequently used calcium antagonist, verapamil hydrochloride. The results of HPLC analysis, supported by both nuclear magnetic resonance and mass spectroscopy studies, showed that frankiamide has a high affinity for Na(+) ions.

• Authors: J P Haansuu, K D Klika, P P Söderholm, V V Ovcharenko, K Pihlaja, K K Haahtela, P M Vuorela
• Journal: Journal of industrial microbiology & biotechnology (J Ind Microbiol Biotechnol) Vol. 27 Issue 1 Pg. 62-6 (Jul 2001) ISSN: 1367-5435 [Print] Germany
• PMID: 11598813 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amides
• Heterocyclic Compounds
• Imides
• frankiamide
• Calcium

Topics

• Actinomycetales (growth & development, metabolism)
• Amides (chemistry, isolation & purification, pharmacology)
• Animals
• Calcium (antagonists & inhibitors, metabolism)
• Cells, Cultured
• Fungi (drug effects)
• Gram-Positive Bacteria (drug effects)
• Heterocyclic Compounds (chemistry, isolation & purification, pharmacology)
• Imides (chemistry, isolation & purification, pharmacology)
• Microbial Sensitivity Tests
• Pituitary Gland (cytology)
• Rats