Identification of the hemolysis-associated protein 1 as a cross-protective immunogen of Leptospira interrogans by adenovirus-mediated vaccination.

Abstract

New vaccine strategies are needed for the prevention of leptospirosis, a widespread human and animal disease caused by pathogenic leptospires. Our previous work determined that a protein leptospiral extract conferred cross-protection in a gerbil model of leptospirosis. The 31- to 34-kDa protein fraction of Leptospira interrogans serovar autumnalis was shown sufficient for this purpose. In the present study, N-terminal sequencing of a 32-kDa fraction and Southern blotting of genomic DNA with corresponding degenerated oligonucleotide probes identified two of its constituents: hemolysis-associated protein 1 (Hap1) and the outer membrane Leptospira protein 1 (OmpL1). Adenovirus-mediated Hap1 vaccination induces significant protection against a virulent heterologous Leptospira challenge in gerbils, whereas a similar OmpL1 construct failed to protect the animals. These data indicate that Hap1 could be a good candidate for developing a new generation of vaccines able to induce broad protection against leptospirosis disease.

• Authors: C Branger, C Sonrier, B Chatrenet, B Klonjkowski, N Ruvoen-Clouet, A Aubert, G André-Fontaine, M Eloit
• Journal: Infection and immunity (Infect Immun) Vol. 69 Issue 11 Pg. 6831-8 (Nov 2001) ISSN: 0019-9567 [Print] United States
• PMID: 11598056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Proteins
• Bacterial Vaccines
• DNA, Bacterial
• Hemolysin Proteins
• Recombinant Fusion Proteins
• Vaccines, Synthetic
• hemolysis-associated protein 1

Topics

• Adenoviridae
• Amino Acid Sequence
• Animals
• Antibodies, Bacterial (immunology)
• Antigens, Bacterial (biosynthesis, genetics, immunology, isolation & purification)
• Bacterial Proteins (biosynthesis, genetics, immunology, isolation & purification)
• Bacterial Vaccines (biosynthesis, genetics, immunology, isolation & purification)
• Base Sequence
• Chemical Fractionation
• Cross Reactions
• DNA, Bacterial
• Genetic Vectors
• Gerbillinae
• Hemolysin Proteins
• Hemolysis
• Leptospira interrogans (immunology)
• Molecular Sequence Data
• Recombinant Fusion Proteins (biosynthesis, genetics, isolation & purification)
• Vaccination
• Vaccines, Synthetic (biosynthesis, genetics, immunology, isolation & purification)
• Weil Disease (prevention & control)