Abstract |
Macrophage-inflammatory protein 2 (MIP-2) is a major CXC chemokine involved in the migration of polymorphonuclear neutrophils (PMNs) to sites of inflammation. Although cell culture experiments have identified different cell types that can produce MIP-2, the cellular sources in vivo are not clearly defined. By using immunohistochemical staining and analysis of chemokine mRNA expression, the present study aimed to localize cells producing MIP-2 in tissues of normal mice and mice challenged with Yersinia enterocolitica. The results showed a constitutive expression of MIP-2 mRNA in bone marrow (BM) of normal mice, but not in other organs such as spleen, lung, or liver. MIP-2 protein was found in all organs tested but it was exclusively associated with PMNs that stained positive with the cell surface marker Gr-1. Bacterial infection caused a 5-fold increase in the number of MIP-2-positive PMNs recruited to spleens concomitant with a strong increase of splenic MIP-2 mRNA. This correlated well with a 3-fold loss of MIP-2-producing cells in BM. Because MIP-2 mRNA expression in PMNs was increased after stimulation with TNF, the results indicate that newly recruited PMNs can supplement their MIP-2 content through TNF-stimulated transcription. Together, the data imply a constitutive production of MIP-2 by a subset of PMNs in BM and argue for the possibility of a rapid mobilization of MIP-2 through its storage in circulating PMNs.
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Authors | S P Matzer, T Baumann, N W Lukacs, M Röllinghoff, H U Beuscher |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 167
Issue 8
Pg. 4635-43
(Oct 15 2001)
ISSN: 0022-1767 [Print] United States |
PMID | 11591793
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokine CXCL2
- Chemokines
- Cxcl2 protein, mouse
- Interleukin-1
- RNA, Messenger
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Bone Marrow Cells
(chemistry, immunology)
- Chemokine CXCL2
- Chemokines
(biosynthesis, genetics, isolation & purification)
- Chemotaxis, Leukocyte
- Female
- Granulocytes
(chemistry)
- Interleukin-1
(pharmacology)
- Leukopoiesis
- Mice
- Mice, Inbred BALB C
- Neutrophils
(chemistry, immunology)
- RNA, Messenger
(isolation & purification)
- Spleen
(cytology, immunology)
- Tissue Distribution
- Tumor Necrosis Factor-alpha
(pharmacology)
- Yersinia Infections
(immunology)
- Yersinia enterocolitica
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