Brain enriched hyaluronan binding (BEHAB)/brevican increases aggressiveness of CNS-1 gliomas in Lewis rats.

Abstract

Gliomas are the most common primary intracranial tumors. One extracellular matrix component that has been implicated in glial tumor biology is brain enriched hyaluronan binding (BEHAB)/brevican. In this study, the CNS-1 rat glioma cell line was transfected with a vector containing either a full-length BEHAB/brevican cDNA, a 5' insert encoding the NH(2)-terminal BEHAB/brevican cleavage product, or a 3' insert encoding the COOH-terminal cleavage product. As a control, CNS-1 cells were transfected with green fluorescent protein. Rats with intracranial grafts of BEHAB/brevican-transfected CNS-1 cells displayed significantly shorter survival times than did rats with CNS-green fluorescent protein intracranial grafts (P < 0.001). Histological examination showed that the BEHAB/brevican-transfected tumors were just as, if not more, aggressive than control tumors, even though the BEHAB/brevican tumors had been growing for only approximately two-thirds the time as long as control tumors. These data suggest that up-regulation and proteolytic cleavage of BEHAB/brevican increase significantly the aggressiveness of glial tumors. It will be important to investigate the effect of inhibiting cleavage of BEHAB/brevican in these cells and to determine the therapeutic potential of inhibiting BEHAB/brevican cleavage in gliomas.

• Authors: C L Nutt, C A Zerillo, G M Kelly, S Hockfield
• Journal: Cancer research (Cancer Res) Vol. 61 Issue 19 Pg. 7056-9 (Oct 01 2001) ISSN: 0008-5472 [Print] United States
• PMID: 11585735 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bcan protein, rat
• Brevican
• Carrier Proteins
• Chondroitin Sulfate Proteoglycans
• Lectins, C-Type
• Nerve Tissue Proteins

Topics

• Animals
• Brain Neoplasms (genetics, metabolism, pathology)
• Brevican
• Carrier Proteins (biosynthesis, genetics, physiology)
• Cell Division (genetics)
• Chondroitin Sulfate Proteoglycans (biosynthesis, genetics, physiology)
• Glioma (genetics, metabolism, pathology)
• Lectins, C-Type
• Nerve Tissue Proteins (biosynthesis, genetics, physiology)
• Rats
• Rats, Inbred Lew
• Transfection
• Up-Regulation