Abstract | PURPOSE: To examine the impact of experimental ischemia and interruption of glutamate transport on retinal neuronal cell, especially retinal ganglion cell (RGC), survival in vitro. METHODS: RESULTS: Control normoxic cultures contained large numbers of immunocytochemically identified photoreceptors (PRs), bipolar cells (BCs), amacrine cells (ACs), and RGCs after 7 days in vitro. A 24-hour postmortem delay before culture led to significant reductions in all types (40%-70%), proportionately greater in ACs and RGCs. Lowering of sugar levels also led to increased losses in all cell types, whereas potassium cyanide treatment deleteriously affected only ACs and RGCs. Ambient hypoxia led to consistent reductions only in the number of RGCs, which were exacerbated by addition of high concentrations of glutamate. Inclusion of glutamate receptor antagonists had a partial protective effect against RGC loss. Treatment with tPDC and THA also led to selective RGC death, but MS had no effect on any cells. CONCLUSIONS: Different components of the ischemic pathologic process ( hypoxia, hypoglycemia, glutamate transport failure) lead to distinctly different patterns of neuronal loss in adult retina in vitro. RGCs are especially vulnerable, corresponding to their in vivo susceptibility. These data may suggest neuroprotective strategies for limiting retinal damage during ischemia.
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Authors | X Luo, G N Lambrou, J A Sahel, D Hicks |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 42
Issue 11
Pg. 2695-705
(Oct 2001)
ISSN: 0146-0404 [Print] United States |
PMID | 11581218
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATP-Binding Cassette Transporters
- Amino Acid Transport System X-AG
- Enzyme Inhibitors
- Eye Proteins
- Nerve Tissue Proteins
- Neurotransmitter Uptake Inhibitors
- Glutamic Acid
- Glutamate-Ammonia Ligase
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Topics |
- ATP-Binding Cassette Transporters
(antagonists & inhibitors)
- Amino Acid Transport System X-AG
- Animals
- Biological Transport
- Cell Death
- Cells, Cultured
- Enzyme Inhibitors
(pharmacology)
- Eye Proteins
(metabolism)
- Fluorescent Antibody Technique, Indirect
- Glutamate-Ammonia Ligase
(antagonists & inhibitors)
- Glutamic Acid
(metabolism)
- Hypoglycemia
(complications)
- Hypoxia
(complications)
- Ischemia
(complications)
- Nerve Tissue Proteins
(metabolism)
- Neurons
(metabolism, pathology)
- Neurotransmitter Uptake Inhibitors
(pharmacology)
- Retinal Diseases
(complications)
- Retinal Ganglion Cells
(metabolism, pathology)
- Retinal Vessels
(pathology)
- Swine
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