Abstract | BACKGROUND: METHODS: BAL, exposed or not to different concentrations of CLS (1.5-30 mM), was assayed for free radical content by fluorometric analysis of DNA unwinding (FADU) or by cytochrome c reduction kinetics. Human serum was treated with ultrasound in the presence or absence of CLS (1.5, 2.5 mM) or N-acetyl cysteine (NAC; 4, 5 mM) and assayed for free radical content by FADU. Human endothelial cells cultured in vitro from pulmonary artery were incubated with elastase (0.3 IU/mL), in the presence or absence of glutathione (GSH; 0.65 mM) or CLS (0.16 mM). The supernatant was tested for cytochrome c reduction kinetics whereas cell homogenates were assessed for xanthine oxidase (XO) content by SDS-PAGE. RESULTS: Results showed that CLS is more effective as an in vitro scavenger in comparison to GSH and NAC. CLS reduced the damage of DNA from healthy donors exposed to COPD-BAL and was able to quench clastogenic activity induced in human serum by exposure to ultrasound at concentrations as low as 2.5 mM. NAC protect DNA from radical damage, starting from 5 mM. In human lung endothelial cells cultured in presence of elastase, CLS (0.16 mM) decreased xanthine oxidase activity. CONCLUSIONS:
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Authors | S Pinamonti, L Venturoli, M Leis, M Chicca, A Barbieri, S Sostero, F Ravenna, L Daffonchio, R Novellini, A Ciaccia |
Journal | Panminerva medica
(Panminerva Med)
Vol. 43
Issue 3
Pg. 215-20
(Sep 2001)
ISSN: 0031-0808 [Print] Italy |
PMID | 11579333
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- carbocysteine-lysine
- Carbocysteine
- Glutathione
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Topics |
- Aged
- Aged, 80 and over
- Antioxidants
(pharmacology)
- Carbocysteine
(analogs & derivatives, pharmacology)
- Cells, Cultured
- DNA Damage
- Female
- Glutathione
(pharmacology)
- Humans
- Male
- Middle Aged
- Pulmonary Disease, Chronic Obstructive
(metabolism)
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