Abstract |
To determine whether treatment with melatonin (MLT) improves the efficiency of immunization against Venezuelan equine encephalomyelitis (VEE) virus, mice were vaccinated with TC-83 VEE virus and treated daily with MLT (1 or 5 mg/kg) starting 3 days before immunization, until 10 days after. IgM antibody titers were determined at days 7, 14, and 21 post-immunization. IL-10 levels were assayed at day 14 postvaccination. Treatment with MLT increased antibody titers 14 days after the immunization. IL-10 levels also increased with MLT treatment (1 and 5 mg/kg). Mice were challenged with live VEE virus at day 21 postimmunization, and viral titers were plaque assayed in chicken embryo fibroblasts 4 days after the infection. Following this challenge brain virus levels were significantly reduced. The results suggest that MLT treatment enhances the efficiency of mice immunization against VEE virus.
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Authors | B Negrette, E Bonilla, N Valero, H Pons, J Garcia Tamayo, L Chacín-Bonilla, S Medina-Leendertz, F Añez |
Journal | Neurochemical research
(Neurochem Res)
Vol. 26
Issue 7
Pg. 767-70
(Jul 2001)
ISSN: 0364-3190 [Print] United States |
PMID | 11565607
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Viral
- Viral Vaccines
- Melatonin
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Animals
- Antibodies, Viral
(analysis)
- Brain
(virology)
- Encephalitis Virus, Venezuelan Equine
(immunology, isolation & purification)
- Encephalomyelitis, Venezuelan Equine
(prevention & control, virology)
- Immunization
- Melatonin
(pharmacology)
- Mice
- Viral Vaccines
(immunology)
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