Melatonin treatment enhances the efficiency of mice immunization with Venezuelan equine encephalomyelitis virus TC-83.

Abstract

To determine whether treatment with melatonin (MLT) improves the efficiency of immunization against Venezuelan equine encephalomyelitis (VEE) virus, mice were vaccinated with TC-83 VEE virus and treated daily with MLT (1 or 5 mg/kg) starting 3 days before immunization, until 10 days after. IgM antibody titers were determined at days 7, 14, and 21 post-immunization. IL-10 levels were assayed at day 14 postvaccination. Treatment with MLT increased antibody titers 14 days after the immunization. IL-10 levels also increased with MLT treatment (1 and 5 mg/kg). Mice were challenged with live VEE virus at day 21 postimmunization, and viral titers were plaque assayed in chicken embryo fibroblasts 4 days after the infection. Following this challenge brain virus levels were significantly reduced. The results suggest that MLT treatment enhances the efficiency of mice immunization against VEE virus.

• Authors: B Negrette, E Bonilla, N Valero, H Pons, J Garcia Tamayo, L Chacín-Bonilla, S Medina-Leendertz, F Añez
• Journal: Neurochemical research (Neurochem Res) Vol. 26 Issue 7 Pg. 767-70 (Jul 2001) ISSN: 0364-3190 [Print] United States
• PMID: 11565607 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Viral
• Viral Vaccines
• Melatonin

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Antibodies, Viral (analysis)
• Brain (virology)
• Encephalitis Virus, Venezuelan Equine (immunology, isolation & purification)
• Encephalomyelitis, Venezuelan Equine (prevention & control, virology)
• Immunization
• Melatonin (pharmacology)
• Mice
• Viral Vaccines (immunology)