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An immediate endothelial cell signaling response to lung ischemia.

Abstract
Abrupt cessation of lung perfusion induces a rapid endothelial response that is not associated with anoxia but reflects loss of normal shear stress. This response includes membrane depolarization, H(2)O(2) generation, and increased intracellular Ca(2+). We evaluated these parameters immediately upon nonhypoxic ischemia using fluorescence videomicroscopy to image in situ endothelial cells in isolated, ventilated rat lungs. Lungs labeled with 4-(2-[6-(dioctylamino)-2-naphthalenyl]ethenyl)1-(3-sulfopropyl)-pyridinium (di-8-ANEPPS; a membrane potential probe), Amplex Red (an extracellular H(2)O(2) probe), or fluo 3-AM (a Ca(2+) indicator) were subjected to control perfusion followed by global ischemia. Endothelial di-8-ANEPPS fluorescence increased significantly within the first second of ischemia and stabilized at 15 s, indicating membrane depolarization by approximately 17 mV; depolarization was blocked by preperfusion with the K(+) channel agonist lemakalim. Increased H(2)O(2), inhibitable by catalase, was detected in the vascular space at 1-2 s after the onset of ischemia. Increased intracellular Ca(2+) was detected 10-15 s after the onset of ischemia; the initial increase was inhibited by preperfusion with thapsigargin. Thus the temporal sequence of the initial response of endothelial cells in situ to loss of shear stress (i.e., ischemia) is as follows: membrane depolarization, H(2)O(2) release, and increased intracellular Ca(2+).
AuthorsC Song, A B Al-Mehdi, A B Fisher
JournalAmerican journal of physiology. Lung cellular and molecular physiology (Am J Physiol Lung Cell Mol Physiol) Vol. 281 Issue 4 Pg. L993-1000 (Oct 2001) ISSN: 1040-0605 [Print] United States
PMID11557603 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • 1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine
  • Fluorescent Dyes
  • Pyridinium Compounds
  • Potassium
  • Calcium
Topics
  • Animals
  • Calcium (metabolism)
  • Cell Communication (physiology)
  • Endothelium, Vascular (metabolism)
  • Fluorescent Dyes
  • In Vitro Techniques
  • Ischemia (metabolism)
  • Lung (blood supply, metabolism)
  • Male
  • Microcirculation (physiology)
  • Potassium (metabolism)
  • Pyridinium Compounds
  • Rats
  • Rats, Sprague-Dawley
  • Stress, Mechanical

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