Protein kinase C and changes in manganese superoxide dismutase gene expression in cultured glial cells.

Abstract

1. To study the role of protein kinase C (PKC) in the increase in manganese superoxide dismutase (Mn-SOD) gene expression following transient hypoxia in glial cells, we examined the mRNA levels of Mn-SOD using northern blot analysis. 2. The Mn-SOD mRNA levels were markedly increased after exposure to nitrogen gas for 5 min. 3. Pretreatment with chelerythrine or GF109203x, inhibitors of PKC, attenuated the increase in Mn-SOD mRNA following hypoxia in a concentration-dependent manner. 4. Incubation with phorbol 12-myristate 13-acetate, the PKC activator, enhanced the increase in Mn-SOD gene expression in response to transient hypoxia. 5. The results suggest that hypoxia increases Mn-SOD gene expression in cultured glial cells mainly through activation of a PKC pathway.

• Authors: W C Huang, P C Chen, S B Jou, J T Cheng
• Journal: Clinical and experimental pharmacology & physiology (Clin Exp Pharmacol Physiol) Vol. 28 Issue 10 Pg. 822-5 (Oct 2001) ISSN: 0305-1870 [Print] Australia
• PMID: 11553022 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• RNA, Messenger
• Superoxide Dismutase
• Protein Kinase C

Topics

• Cell Hypoxia (drug effects, physiology)
• Cells, Cultured
• Enzyme Inhibitors (pharmacology)
• Gene Expression Regulation
• Neuroglia (drug effects, enzymology)
• Protein Kinase C (biosynthesis)
• RNA, Messenger (biosynthesis)
• Superoxide Dismutase (biosynthesis, genetics)