Abstract |
Glucocorticoid has long been used to treat patients with glomerulonephritis because it ameliorates mesangial cell proliferation and proteinuria, in part by suppressing nuclear factor-kappa B ( NF-kappaB) activation, which regulates the transcription of various pro-inflammatory genes. Recent evidence shows that NF-kappaB activation increases the resistance to TNF-alpha-induced apoptosis in mesangial cells. We examined glomerular cell proliferation and apoptosis along with NF-kappaB activation in the Thy-1.1 nephritis model. We also evaluated TNF-alpha-induced apoptosis in cultured mesangial cells. Methylprednisolone treatment ameliorated mesangial hypercellularity in Thy-1.1 nephritis by decreasing proliferating cells and increasing apoptosis in the glomeruli. These effects were associated with suppressed NF-kappaB activation. This in vitro study revealed that treatment with methylprednisolone and TNF-alpha induced cultured mesangial cell apoptosis. These results suggest that methylprednisolone may accelerate the resolution phase of Thy-1.1 nephritis in part by sensitizing mesangial cells to apoptosis.
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Authors | K Maruyama, N Kashihara, Y Yamasaki, M Sato, H Sugiyama, K Okamoto, Y Maeshima, M Odawara, J Sasaki, H Makino |
Journal | Experimental nephrology
(Exp Nephrol)
Vol. 9
Issue 5
Pg. 317-26
( 2001)
ISSN: 1018-7782 [Print] Switzerland |
PMID | 11549849
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2001 S. Karger AG, Basel |
Chemical References |
- Glucocorticoids
- NF-kappa B
- RNA, Messenger
- Tumor Necrosis Factor-alpha
- Methylprednisolone
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Topics |
- Animals
- Apoptosis
- Cells, Cultured
- Disease Models, Animal
- Glomerular Mesangium
(drug effects, pathology)
- Glomerulonephritis
(drug therapy, pathology)
- Glucocorticoids
(therapeutic use)
- Immunohistochemistry
- In Situ Nick-End Labeling
- Male
- Methylprednisolone
(therapeutic use)
- NF-kappa B
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Wistar
- Tumor Necrosis Factor-alpha
(pharmacology)
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