Abstract |
Expression of MMP-2 in melanoma cells has been demonstrated to be involved in the degradation of extracellular matrix during melanoma growth and to correlate with later melanoma metastasis. MMP-2 is considered to be activated by membrane-associated matrix metalloproteinases (MT- MMPs). To know whether MT- MMPs are involved in the activation of MMP-2 in melanoma cells, immunohistochemical studies were performed in primary and metastatic melanoma by use of the antibodies for MT1-MMP, MT2-MMP and MT3-MMP. Expression of MT1-MMP, MT2-MMP, MT3-MMP and MMP-2 in nevocellular nevus (n = 5), dysplastic nevus (n = 2) and juvenile melanoma (n = 3) was undetectable or detected in only a few cells. Superficial spreading melanoma (SSM) (n = 3) and acral lentiginous melanoma (ALM) (n = 3) showed a moderate expression of MT1 approximately 3-MMP. In nodular melanoma (NM) (n = 2) and metastatic melanoma (n = 3), MT1 approximately 3-MMP was more intensely expressed. Double immunofluorescence demonstrated a consistent colocalization of MT2- MMP/ MMP-2 and MT3- MMP/ MMP-2 in the NM and metastatic melanoma cells. The colocalization of MT2,3-MMP and MMP-2 in nodular and metastatic melanoma cells suggests that MT- MMPs and MMP-2 co-operate in the invasive and metastatic process of melanoma cells.
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Authors | Y Ohnishi, S Tajima, A Ishibashi |
Journal | European journal of dermatology : EJD
(Eur J Dermatol)
2001 Sep-Oct
Vol. 11
Issue 5
Pg. 420-3
ISSN: 1167-1122 [Print] France |
PMID | 11525948
(Publication Type: Journal Article)
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Chemical References |
- MMP15 protein, human
- MMP16 protein, human
- Matrix Metalloproteinase 15
- Matrix Metalloproteinase 16
- Matrix Metalloproteinases, Membrane-Associated
- Metalloendopeptidases
- Matrix Metalloproteinase 2
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Topics |
- Humans
- Immunohistochemistry
- Matrix Metalloproteinase 15
- Matrix Metalloproteinase 16
- Matrix Metalloproteinase 2
(biosynthesis)
- Matrix Metalloproteinases, Membrane-Associated
- Melanoma
(enzymology, pathology)
- Metalloendopeptidases
(biosynthesis)
- Neoplasm Metastasis
- Skin
(enzymology, pathology)
- Skin Neoplasms
(enzymology, pathology)
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