Dendritic cells transduced with HIV Nef express normal levels of HLA-A and HLA-B class I molecules.

Abstract

HIV Nef protein is important for viral pathogenesis and disease progression. Nef downregulates CD4 and major histocompatibility antigens on the surface of HIV-infected T cells. HIV also infects dendritic cells. We wanted to determine if Nef had a similar function in professional antigen-presenting cells, where downregulation of Class I could have important effects on the initiation of HIV specific cytolytic T cell responses. We infected human dendritic cells with adenovirus expressing Nef. In contrast to T cells and Hela cells, HLA-A and HLA-B molecules are not downregulated nor are other class I molecules increased. We show that, in dendritic cells, HIV Nef has little effect on CD4 or Class I expression.

• Authors: L A Cramer, J A Frelinger
• Journal: Journal of acquired immune deficiency syndromes (1999) (J Acquir Immune Defic Syndr) Vol. 27 Issue 5 Pg. 417-25 (Aug 15 2001) ISSN: 1525-4135 [Print] United States
• PMID: 11511817 (Publication Type: Journal Article)

Chemical References

• CD4 Antigens
• Gene Products, nef
• HLA-A Antigens
• HLA-B Antigens
• Luminescent Proteins
• nef Gene Products, Human Immunodeficiency Virus
• Green Fluorescent Proteins

Topics

• Adenoviridae (genetics, metabolism)
• CD4 Antigens (metabolism)
• Cells, Cultured
• Dendritic Cells (immunology, metabolism, virology)
• Gene Products, nef (metabolism)
• Genes, nef
• Green Fluorescent Proteins
• HIV-1 (metabolism)
• HLA-A Antigens (metabolism)
• HLA-B Antigens (metabolism)
• HeLa Cells
• Humans
• Luminescent Proteins (genetics, metabolism)
• Transduction, Genetic
• nef Gene Products, Human Immunodeficiency Virus