Choriocarcinoma cells modulate the cytokine production of decidual large granular lymphocytes in coculture.

Abstract

PROBLEM:

How do major histocompatibility complex (MHC) I positive/negative choriocarcinoma (CC) cells affect the production of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-10 (IL-10), interleukin-4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF) by decidual CD56++ cells (large granular lymphocytes [LGL]), LGL-depleted decidual cells (WASH) and unseparated decidual cells (DEC) in cocultures?

METHOD OF STUDY:

Decidual tissue was obtained by legal abortions. CD 56++ LGL were isolated in a magnetic cell separator. Cytokines were measured by ELISA. Differences were analyzed for significance by Wilcoxons test.

RESULTS:

We found a significant increase of IL-10 in LGL/JEG-3 and of TNF-alpha in LGL/JAR cocultures compared to noncocultured decidual cells. There was a significant increase of IL-10 and TNF-alpha and a significant decrease of GM-CSF in WASH and DEC cocultures with both JEG-3 and JAR. IFN-gamma was only found in 3/11 cases of LGL/JAR cocultures. No IL-4 was found in any experiment.

CONCLUSION:

MHC class I positive/negative CC modulate the cytokine production of decidual LGL in different ways.

• Authors: L Rieger, U Kämmerer, J Hofmann, M Sütterlin, J Dietl
• Journal: American journal of reproductive immunology (New York, N.Y. : 1989) (Am J Reprod Immunol) Vol. 46 Issue 2 Pg. 137-43 (Aug 2001) ISSN: 1046-7408 [Print] Denmark
• PMID: 11506078 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Cytokines
• Histocompatibility Antigens Class I

Topics

• Cell Fractionation
• Cells, Cultured
• Choriocarcinoma (immunology)
• Coculture Techniques (methods)
• Culture Media
• Cytokines (biosynthesis)
• Decidua (cytology, immunology)
• Female
• Flow Cytometry (methods)
• Histocompatibility Antigens Class I (immunology)
• Humans
• Lymphocytes (immunology)
• Pregnancy
• Trophoblasts (cytology, immunology)
• Tumor Cells, Cultured