Abstract | OBJECTIVE: DESIGN: Prospective, randomized trial. SETTING: Animal research center. SUBJECTS: Male Sprague-Dawley rats. INTERVENTIONS: MEASUREMENTS AND MAIN RESULTS: To obtain tissues for assessing mucosal permeability and histology, five 10-cm long segments of small intestine were obtained at the following time points: baseline, after 30 and 60 mins of ischemia, and after 30 and 60 mins of reperfusion. Mucosal permeability to fluorescein isothiocyanate dextran (molecular weight 4000 Da) was assessed ex vivo by using an everted gut sac method. Compared with controls, treatment of rats with either pyruvate solution or ethyl pyruvate solution significantly ameliorated the development of intestinal mucosal hyperpermeability during the reperfusion. Treatment with ethyl pyruvate solution also significantly decreased the extent of histologic mucosal damage after mesenteric reperfusion. CONCLUSIONS:
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Authors | C A Sims, S Wattanasirichaigoon, M J Menconi, A M Ajami, M P Fink |
Journal | Critical care medicine
(Crit Care Med)
Vol. 29
Issue 8
Pg. 1513-8
(Aug 2001)
ISSN: 0090-3493 [Print] United States |
PMID | 11505117
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Mutagens
- Pyruvates
- ethyl pyruvate
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Topics |
- Animals
- Intestinal Mucosa
(blood supply, injuries)
- Intestine, Small
(pathology)
- Ischemia
(prevention & control)
- Male
- Mutagens
(therapeutic use)
- Pyruvates
(therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
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