Simultaneous determination of F-beta-alanine and beta-alanine in plasma and urine with dual-column reversed-phase high-performance liquid chromatography.

Abstract

F-beta-Alanine and beta-alanine were detected in plasma and urine samples with fluorescence detection of orthophthaldialdehyde derivatives of F-beta-alanine and beta-alanine after separation with dual-column reversed-phase HPLC. The detection limits of F-beta-alanine and beta-alanine in the HPLC system were approximately 0.3 and 0.7 pmol, respectively. The procedure proved to be very reproducible with intra-assay RSDs and inter-assay RSDs being less than 8%. The usefulness of the method was demonstrated by the analysis of the F-beta-alanine and beta-alanine concentrations in plasma and urine samples from tumor patients treated with S-1 (Tegafur, 5-chloro-2,4-dihydroxypyridine and potassium oxonate in a molar ratio of 1:0.4:1).

• Authors: A B Van Kuilenburg, A E Stroomer, G J Peters, A H Van Gennip
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 759 Issue 1 Pg. 51-61 (Aug 05 2001) ISSN: 1387-2273 [Print] Netherlands
• PMID: 11499629 (Publication Type: Journal Article)

Chemical References

• beta-Alanine
• Tegafur

Topics

• Chromatography, High Pressure Liquid (methods)
• Humans
• Neoplasms (blood, drug therapy, urine)
• Reproducibility of Results
• Spectrometry, Fluorescence
• Tegafur (administration & dosage, pharmacokinetics, therapeutic use)
• beta-Alanine (analysis, blood, urine)