Expression of the matrix metalloproteinase promatrilysin in coculture of prostate carcinoma cell lines.

Abstract

BACKGROUND:

Matrix metalloproteinases (MMPs) are involved in tumor progression. Matrilysin (MMP-7) has been shown to be upregulated in prostatic carcinomas and can increase the invasive capacity of DU-145 cells. Because of the heterogenous nature of prostatic tumors, we examined promatrilysin expression in cocultures containing two different prostatic carcinoma cell lines, DU-145 and LNCaP.

METHODS:

Using enzyme linked immunosorbent assay (ELISA) analyses, promatrilysin expression was measured in DU-145/LNCaP cocultures and conditioned media cross-cultures. The effects of blocking IL-6 on promatrilysin expression were examined by pretreating conditioned media with IL-6 neutralizing antibody.

RESULTS:

A significant induction of promatrilysin expression was observed in DU-145/LNCaP cocultures compared to LNCaP cells alone. In addition, DU-145 conditioned medium induced the same fold induction of promatrilysin as was observed in the cocultures. LNCaP cell conditioned medium did not induce promatrilysin expression in DU-145 cells. Neutralization of IL-6 with neutralizing antibody abrogated DU-145 conditioned media induced promatrilysin expression to baseline levels.

CONCLUSIONS:

IL-6 secreted by DU-145 cells can induce promatrilysin expression in LNCaP cells. IL-6, in vivo, may act as a paracrine signaling factor that regulates matrix metalloproteinase expression. Therefore, IL-6 may play a role in invasive metastatic processes of a prostate carcinoma.

• Authors: M S Stratton, H Sirvent, T S Udayakumar, R B Nagle, G T Bowden
• Journal: The Prostate (Prostate) Vol. 48 Issue 3 Pg. 206-9 (Aug 01 2001) ISSN: 0270-4137 [Print] United States
• PMID: 11494336 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 2001 Wiley-Liss, Inc.

Chemical References

• Interleukin-6
• Matrix Metalloproteinase 7

Topics

• Carcinoma (genetics, pathology)
• Enzyme-Linked Immunosorbent Assay
• Gene Expression Regulation, Neoplastic
• Humans
• Interleukin-6 (pharmacology)
• Male
• Matrix Metalloproteinase 7 (biosynthesis)
• Neoplasm Invasiveness
• Prostatic Neoplasms (genetics, pathology)
• Signal Transduction
• Tumor Cells, Cultured