Abstract | BACKGROUND: Previous studies suggest that CD8(+) T cells are immunosuppressive after burn injury, but recent reports indicate that CD8(+) T cells have several functions similar to CD4(+) T cells, including the secretion of cytokines. This study uses HY male antigen in transgenic HY female mice to determine the antigen-specific response of activated CD8(+) T cells after burn injury. METHODS: HY TCR transgenic female mice underwent burn or sham injury. Seventy-two hours after the burn, splenocytes were stimulated with 20 micromol/L HY peptide for 16, 48, and 64 hours; cellular proliferation, intracellular interferon-gamma and interleukin-2, and apoptosis were measured. RESULTS:
Burn injury significantly impaired proliferation to HY antigen (P < or =.05). Activated CD8(+) T cells from burned mice showed increased intracellular interferon-gamma and interleukin-2 16 hours after stimulation compared with sham (P < or =.05) and at no time was less than control mice. The percent of CD8(+) T cells decreased with the time of stimulation but was not due to apoptosis by Annexin V staining. CONCLUSIONS: Activated CD8(+) T cells express a T(h1)-like phenotype after burn injury. This provides evidence that CD8(+) T cells are not simply suppressive and that is consistent with data that CD4(+) T cells are primed for a T(h1) response after burn injury.
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Authors | B A Cairns, R Maile, I Buchanan, D Pilati, S DeSerres, E J Collins, J A Frelinger, A A Meyer |
Journal | Surgery
(Surgery)
Vol. 130
Issue 2
Pg. 210-6
(Aug 2001)
ISSN: 0039-6060 [Print] United States |
PMID | 11490351
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Receptors, Antigen, T-Cell
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Topics |
- Animals
- Apoptosis
(immunology)
- Burns
(immunology)
- CD8-Positive T-Lymphocytes
(cytology, immunology)
- Cells, Cultured
- Female
- Flow Cytometry
- Immunophenotyping
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Receptors, Antigen, T-Cell
(genetics, immunology)
- Spleen
(cytology, immunology)
- Th1 Cells
(cytology, immunology)
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