[Study on the molecular mechanisms involved in the increased collagen synthesis by platelet-derived wound healing factors during wound healing in alloxan-induced diabetic rat].

Abstract

OBJECTIVE:

To explore the molecular mechanisms involved in the increased collagen synthesis by platelet-derived wound healing factors (PDWHF) during wound healing in alloxan-induced diabetic rats.

METHODS:

Thirty-three male SD rats were divided into two groups, the normal (n = 9) (group A) and the diabetic group (n = 24). Two pieces of full-thickness skin with diameter of 1.8 cm were removed from the dorsal site of diabetic rats. PDWHF (100 micrograms/wound) was topically applied to one side of the diabetic wounds (group B) on the operation day and then once a day in the next successive 6 days. Meanwhile, bovine serum albumin (100 micrograms/wound) was applied to the other side of diabetic wound as control group (group C) in the same way. Levels of transforming growth factor-beta 1 (TGF-beta 1) and procollagen I mRNA in wound tissue were inspected by dot blotting.

RESULTS:

TGF-beta 1 mRNA levels in group B were 4 folds and 5.6 folds compared with those in group C after 5 and 7 days (P < 0.01), however, still significantly lower than those of group A (P < 0.05). There was no significance difference among three groups on the 10th day after wounding. The levels for procollagen I mRNA in group B amounted to 2.1, 1.8 and 2.3 folds of those in group C after 5, 7, and 10 days (P < 0.01), respectively. Compared with those in the group A, procollagen I mRNA levels in the group B were significantly lower after 5 and 7 days (P < 0.05), and no significant difference was observed between group B and A after 10 days.

CONCLUSION:

One important way for PDWHF to enhance the collagen synthesis in diabetic wound healing is to increase the gene expression of endogenous TGF-beta 1.

• Authors: X D Zhu, Y Zhang, C X Hu
• Journal: Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery (Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi) Vol. 15 Issue 4 Pg. 223-6 (Jul 2001) ISSN: 1002-1892 [Print] China
• PMID: 11488031 (Publication Type: English Abstract, Journal Article)

Chemical References

• CT-102 activated platelet supernatant
• Complex Mixtures
• Growth Substances
• Procollagen
• RNA, Messenger
• Transforming Growth Factor beta

Topics

• Animals
• Complex Mixtures
• Diabetes Mellitus, Experimental (metabolism)
• Growth Substances (pharmacology)
• In Vitro Techniques
• Male
• Procollagen (biosynthesis, genetics)
• RNA, Messenger (biosynthesis)
• Rats
• Rats, Sprague-Dawley
• Transforming Growth Factor beta (biosynthesis, genetics)
• Wound Healing