Abstract | BACKGROUND AND PURPOSE: The seleno-organic compound ebselen has both antioxidant and anti-inflammatory properties. Although ebselen has been shown to protect the brain against stroke, it is unclear how ebselen provides neuroprotection. In the present study the authors examined whether ebselen inhibits neuronal apoptosis resulting from transient focal cerebral ischemia in mice. The cytochrome c release and DNA fragmentation, both of which are biochemical markers of apoptosis, were compared between vehicle- and ebselen-treated mice. METHODS: RESULTS: - Cytochrome c release was detected in the ischemic hemisphere at 3 to 24 hours after ischemia. Ebselen treatment diminished the cytochrome c release at 12 and 24 hours. In addition, ebselen decreased both DNA fragmentation determined by TUNEL and brain damage volume at 3 days after ischemia. Furthermore, ebselen increased the number of NeuN immunopositive cells at 21 days after ischemia. CONCLUSIONS:
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Authors | S Namura, I Nagata, S Takami, H Masayasu, H Kikuchi |
Journal | Stroke
(Stroke)
Vol. 32
Issue 8
Pg. 1906-11
(Aug 2001)
ISSN: 1524-4628 [Electronic] United States |
PMID | 11486124
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Azoles
- Cytochrome c Group
- Isoindoles
- Neuroprotective Agents
- Organoselenium Compounds
- ebselen
- Casp3 protein, mouse
- Caspase 3
- Caspases
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Topics |
- Animals
- Apoptosis
(drug effects)
- Azoles
(pharmacology)
- Blotting, Western
- Brain
(drug effects, metabolism, pathology)
- Caspase 3
- Caspases
(metabolism)
- Cell Survival
(drug effects)
- Cytochrome c Group
(metabolism)
- DNA Fragmentation
(drug effects)
- Disease Models, Animal
- Enzyme Activation
(drug effects)
- In Situ Nick-End Labeling
- Ischemic Attack, Transient
(drug therapy, metabolism, pathology)
- Isoindoles
- Male
- Mice
- Mice, Inbred ICR
- Mitochondria
(drug effects, enzymology, pathology)
- Neuroprotective Agents
(pharmacology)
- Organoselenium Compounds
(pharmacology)
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