Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter.

Abstract

The uptake of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, was studied in PC12 cells. The compound was actively taken up by the PC12 cells, with a Km value of 176.24 +/-9.1 microM and a maximum velocity of 55.6 +/- 7.0 pmol/min per mg protein; norsalsolinol uptake was dependent on the presence of extracellular Na+. The uptake of norsalsolinol was sensitive to two dopamine transporter inhibitors, GBR-12909 and reserpine, but was less sensitive to desipramine, a noradrenaline transporter inhibitor. Dopamine competitively inhibited norsalsolinol uptake into PC12 cells with a Ki value of 271.2 +/- 61.6 microM. These results suggest that norsalsolinol is taken up into PC12 cells mainly by the dopamine transporter.

• Authors: Y Maruyama, Y Suzuki, A Kazusaka, S Fujita
• Journal: Archives of toxicology (Arch Toxicol) Vol. 75 Issue 4 Pg. 209-13 (Jun 2001) ISSN: 0340-5761 [Print] Germany
• PMID: 11482518 (Publication Type: Journal Article)

Chemical References

• Adrenergic Uptake Inhibitors
• Dopamine Plasma Membrane Transport Proteins
• Dopamine Uptake Inhibitors
• Membrane Glycoproteins
• Membrane Transport Proteins
• Nerve Tissue Proteins
• Piperazines
• Salsoline Alkaloids
• Reserpine
• vanoxerine
• norsalsolinol
• Desipramine
• Dopamine

Topics

• Adrenergic Uptake Inhibitors (pharmacology)
• Animals
• Cells, Cultured
• Chromatography, High Pressure Liquid
• Desipramine (pharmacology)
• Dopamine (metabolism)
• Dopamine Plasma Membrane Transport Proteins
• Dopamine Uptake Inhibitors (pharmacology)
• Dose-Response Relationship, Drug
• Membrane Glycoproteins
• Membrane Transport Proteins (metabolism)
• Nerve Tissue Proteins
• PC12 Cells
• Piperazines (pharmacology)
• Rats
• Reserpine (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Salsoline Alkaloids (metabolism, toxicity)