Isolation and NMR characterization of rosacelose, a novel sulfated polysaccharide from the sponge Mixylla rosacea.

Abstract

Rosacelose, a new anti-HIV polysaccharide composed of glucose and fucose sulfate, has been isolated from an aqueous extract of the marine sponge Mixylla rosacea. Extensive use of 1H and 13C multidimensional NMR spectroscopy, combined with chemical analysis were used to establish a linear polysaccharide structure composed mainly of 4,6-disulfated 3-O-glycosylated alpha-D-glucopyranosyl and 2,4-disulfated 3-O-glycosylated alpha-L-fucopyranosyl residues (in a 3:1 molar ratio).

• Authors: P Cimino, G Bifulco, A Casapullo, I Bruno, L Gomez-Paloma, R Riccio
• Journal: Carbohydrate research (Carbohydr Res) Vol. 334 Issue 1 Pg. 39-47 (Aug 03 2001) ISSN: 0008-6215 [Print] Netherlands
• PMID: 11470249 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-HIV Agents
• Polysaccharides
• Sulfuric Acid Esters
• rosacelose

Topics

• Animals
• Anti-HIV Agents (chemistry, isolation & purification)
• Carbohydrate Sequence
• Marine Biology (methods)
• Molecular Sequence Data
• Molecular Weight
• Nuclear Magnetic Resonance, Biomolecular (methods)
• Polysaccharides (chemistry, isolation & purification)
• Porifera (chemistry)
• Sulfuric Acid Esters