Differential gene regulation by 9-cis and all-trans retinoic acid in neuroblastoma cells.

Abstract

BACKGROUND:

9-cis retinoic acid (RA) is more effective than all-trans RA at inducing neuroblastoma differentiation in vitro, and has distinct biological properties with respect to its ability to promote apoptosis in N-type neuroblastoma cells. The cellular effects of 9-cis RA may, in part, result from activation of retinoid X receptor (RXR) homodimers. If this hypothesis is correct, 9-cis RA may control the expression of a different subset of retinoid-regulated genes compared to all-trans RA.

PROCEDURE:

We have therefore used differential mRNA display to identify genes differentially expressed in neuroblastoma cells in response to all-trans and 9-cis RA.

RESULTS:

The majority of cDNAs differentially expressed in response to all-trans or 9-cis RA matched to nonredundant Genbank sequences or EST database sequences. Differential-display profiles were similar in SH SY 5Y and SH S EP cells, clonal derivatives of the mixed neuroblastoma cell line SK N SH, although there were apparent differences between these cell lines with respect to the retinoid-regulation of specific RT-PCR cDNA fragments.

CONCLUSIONS:

These data support the view that 9-cis and all-trans RA act via different receptor mechanisms.

• Authors: P E Lovat, M Dobson, A J Malcolm, A D Pearson, C P Redfern
• Journal: Medical and pediatric oncology (Med Pediatr Oncol) Vol. 36 Issue 1 Pg. 135-8 (Jan 2001) ISSN: 0098-1532 [Print] United States
• PMID: 11464866 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Neoplasm Proteins
• RNA, Messenger
• RNA, Neoplasm
• Receptors, Retinoic Acid
• Alitretinoin
• Tretinoin

Topics

• Alitretinoin
• Apoptosis (drug effects)
• DNA, Complementary (genetics)
• Expressed Sequence Tags
• Gene Expression Profiling (methods)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Neoplasm Proteins (biosynthesis, drug effects, genetics)
• RNA, Messenger (biosynthesis)
• RNA, Neoplasm (biosynthesis)
• Receptors, Retinoic Acid (drug effects, physiology)
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Homology, Nucleic Acid
• Transcription, Genetic (drug effects)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured (drug effects)