Differential gene regulation by 9-cis and all-trans retinoic acid in neuroblastoma cells.

9-cis retinoic acid (RA) is more effective than all-trans RA at inducing neuroblastoma differentiation in vitro, and has distinct biological properties with respect to its ability to promote apoptosis in N-type neuroblastoma cells. The cellular effects of 9-cis RA may, in part, result from activation of retinoid X receptor (RXR) homodimers. If this hypothesis is correct, 9-cis RA may control the expression of a different subset of retinoid-regulated genes compared to all-trans RA.
We have therefore used differential mRNA display to identify genes differentially expressed in neuroblastoma cells in response to all-trans and 9-cis RA.
The majority of cDNAs differentially expressed in response to all-trans or 9-cis RA matched to nonredundant Genbank sequences or EST database sequences. Differential-display profiles were similar in SH SY 5Y and SH S EP cells, clonal derivatives of the mixed neuroblastoma cell line SK N SH, although there were apparent differences between these cell lines with respect to the retinoid-regulation of specific RT-PCR cDNA fragments.
These data support the view that 9-cis and all-trans RA act via different receptor mechanisms.
AuthorsP E Lovat, M Dobson, A J Malcolm, A D Pearson, C P Redfern
JournalMedical and pediatric oncology (Med Pediatr Oncol) Vol. 36 Issue 1 Pg. 135-8 (Jan 2001) ISSN: 0098-1532 [Print] United States
PMID11464866 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Complementary
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Retinoic Acid
  • alitretinoin
  • Tretinoin
  • Apoptosis (drug effects)
  • DNA, Complementary (genetics)
  • Expressed Sequence Tags
  • Gene Expression Profiling (methods)
  • Gene Expression Regulation, Neoplastic (drug effects)
  • Humans
  • Neoplasm Proteins (biosynthesis, drug effects, genetics)
  • RNA, Messenger (biosynthesis)
  • RNA, Neoplasm (biosynthesis)
  • Receptors, Retinoic Acid (drug effects, physiology)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic (drug effects)
  • Tretinoin (pharmacology)
  • Tumor Cells, Cultured (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: