Urease has been suggested to be essential for colonization and pathogenesis of Helicobacter pylori
infection. In the present study, we evaluated the effects of
urease inhibitors [
acetohydroxamic acid (AHA) and
flurofamide (FFA)] on H. pylori-induced
gastritis in Mongolian gerbils. Animals were orally inoculated with H. pylori, and given
urease inhibitors in their diet throughout the experimental period of six weeks or four weeks, starting from two weeks after H. pylori inoculation. With the administration of AHA at doses of 100, 500, and 2500 ppm throughout the experimental period, H. pylori-induced
gastritis in animals was decreased in a dose-dependent manner, significantly so at 2500 ppm. Suppression of gastric lesions was also evident in animals administered 2500 ppm AHA after the H. pylori
infection.
Bacterial infection rates were reduced to 40-50% of the control value of 100%, by the highest dose of AHA. The potent
urease inhibitor, FFA, also caused marked amelioration of H. pylori-associated
gastritis on administration at 100 ppm throughout the six-week experimental period or for four weeks after H. pylori
infection. Animals treated with FFA had few visible gastric lesions, and the proportion infected with H. pylori was reduced to less than 10%. Since
antibiotic-resistant strains of H. pylori have become a serious problem, nonantibiotic
urease inhibitors may be very useful to control H. pylori-associated gastroduodenal disease.