Detection of moderate hyperhomocysteinemia: comparison of the Abbott fluorescence polarization immunoassay with the Bio-Rad and SBD-F high-performance liquid chromatographic assays.

Abstract

The importance of accurate methods for homocysteine measurement has been emphasized. We compared the results obtained with the most commonly used high-performance liquid chromatography (HPLC) assay, and two recently commercially available methods: another HPLC and a fluorescence polarization immunoassay, in plasmas from normo- or hyperhomocysteinemic patients. A significant agreement between the different methods in classifying the results as hyper or normal-homocysteinemia was observed. However, a significant difference between the results was found. Standardization is urgently necessary to improve the concordance of homocysteine determination.

• Authors: F Barbé, I Abdelmouttaleb, A Chango, P Gérard, D Quilliot, M Klein, D Lambert, J P Nicolas, J L Guéant
• Journal: Amino acids (Amino Acids) Vol. 20 Issue 4 Pg. 435-40 ( 2001) ISSN: 0939-4451 [Print] Austria
• PMID: 11452986 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Cysteine

Topics

• Chemistry, Clinical (methods)
• Chromatography, High Pressure Liquid (methods)
• Cysteine (blood)
• Diabetes Mellitus (blood)
• Heart Diseases (blood)
• Humans
• Hyperhomocysteinemia (diagnosis)
• Hyperthyroidism (blood)
• Hypothyroidism (blood)
• Spectrometry, Fluorescence (methods)