Abstract |
Gene sequence analysis of fusion (F) gene cleavage motifs and haemagglutinin- neuraminidase (HN) carboxyl-terminal extension sequences was used to analyse Newcastle disease viruses (NDV) associated with virulent outbreaks of the disease which occurred in New South Wales, Australia in 1998-2000. PCR fragments were amplified directly from diseased tissue or allantoic fluids and sequence analyses used for phylogenetic comparisons between these viruses and Australian reference NDV. F and HN gene sequence comparison showed a strong relationship to sequences derived from endemic Australian NDV rather than those of overseas viruses or wild bird isolates. Prior to notification of the 1998 outbreak, an NDV was isolated from chickens suffering respiratory disease that appeared to be the progenitor virus from which the virulent virus originated. In turn, these viruses are closely related to two previously isolated 'ancestor' viruses that have the same unique HN extension sequence.
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Authors | A R Gould, J A Kattenbelt, P Selleck, E Hansson, A Della-Porta, H A Westbury |
Journal | Virus research
(Virus Res)
Vol. 77
Issue 1
Pg. 51-60
(Sep 2001)
ISSN: 0168-1702 [Print] Netherlands |
PMID | 11451487
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HN Protein
- Viral Fusion Proteins
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Topics |
- Amino Acid Sequence
- Animals
- Australia
(epidemiology)
- Base Sequence
- Birds
- Disease Outbreaks
- HN Protein
(chemistry, genetics)
- Humans
- Molecular Epidemiology
- Molecular Sequence Data
- Newcastle Disease
(epidemiology, virology)
- Newcastle disease virus
(genetics, isolation & purification, pathogenicity)
- Phylogeny
- Sequence Analysis, DNA
- Viral Fusion Proteins
(chemistry, genetics)
- Virulence
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