Butyl acetate is a volatile aroma and flavor compound in apple. Conidia of three strains of Botrytis cinerea, a fungus that causes decay of apple fruit in postharvest storage, had greater adhesion to and greater germination on
polycarbonate membrane filters on water inside sealed 500 cc glass jars that were injected with 4 microliters
butyl acetate than conidia not so exposed. Conidial germination was highly correlated with conidial adhesion. The yeasts Sporobolomyces roseus and Cryptococcus laurentii, but not Saccharomyces cerevisiae, reduced the adhesion and germination promoting effect of
butyl acetate. Conidia did not readily utilize
butyl acetate as a food source, as shown by lack of
tetrazolium violet reduction, whereas S. roseus and C. laurentii, but not S. cerevisiae did.
Butyl acetate added to
suspensions of conidia increased the electrical conductivity of the
suspensions and increased the loss of 14C from 14C-labeled conidia compared to conidia unexposed to
butyl acetate. Uptake of [14C]
glucose by conidia was not increased by
butyl acetate.
Wounds of Golden Delicious apples inoculated with conidia (strain F-J-4) in a dilute
solution of
butyl acetate had greater decay than unexposed
wounds. S. roseus and C. laurentii, but not S. cerevisiae, added with the conidia decreased the incidence or size of decay. Results indicated that
butyl acetate increased conidial adhesion, stimulating conidial germination, and some yeasts can reduce this effect.