Identification of the gene encoding NADH-rubredoxin oxidoreductase in Clostridium acetobutylicum.

Abstract

NADH-rubredoxin oxidoreductase (NROR), a flavoprotein from the obligately anaerobe Clostridium acetobutylicum is encoded by an ORF (nror) of 1140 nucleotides. Whereas primary structure analysis reveals that NROR has amino acid sequence patterns homologous with those involved in FAD and NAD-binding, the enzyme is distantly related to other flavoproteins in the databank. NROR is highly active for reducing clostridial rubredoxin (Rd) especially against C. acetobutylicum Rd with an efficiency (k(cat)/K(m)) of 400,000 mM(-1)s(-1). These results suggest that Rd from C. acetobutylicum, C. pasteurianum, C. butyricum, and C. cellulolyticum can be interchanged with each other. Since C. acetobutylicum is the sole Clostridium strain that possesses such an enzyme, possible functions are discussed with regard to Desulfovibrio gigas and Pyrococcus furiosus, the only two other anaerobic systems for which a similar activity was reported, but no gene isolated.

• Authors: E Guedon, H Petitdemange
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 285 Issue 2 Pg. 496-502 (Jul 13 2001) ISSN: 0006-291X [Print] United States
• PMID: 11444870 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 Academic Press.

Chemical References

• Recombinant Proteins
• Rubredoxins
• Flavin-Adenine Dinucleotide
• NADP
• Adenosine Diphosphate
• rubredoxin-NAD+ reductase
• NADH, NADPH Oxidoreductases

Topics

• Adenosine Diphosphate (metabolism)
• Amino Acid Sequence
• Anaerobiosis
• Binding Sites
• Clostridium (enzymology, genetics)
• Desulfovibrio (enzymology)
• Flavin-Adenine Dinucleotide (metabolism)
• Kinetics
• Molecular Sequence Data
• Molecular Weight
• NADH, NADPH Oxidoreductases (chemistry, genetics, metabolism)
• NADP (metabolism)
• Open Reading Frames
• Oxidation-Reduction
• Pyrococcus furiosus (enzymology)
• Recombinant Proteins (chemistry, metabolism)
• Rubredoxins (metabolism)
• Sequence Alignment
• Sequence Homology, Amino Acid
• Substrate Specificity