HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

TRAIL (APO-2L) induces apoptosis in human prostate cancer cells that is inhibitable by Bcl-2.

Abstract
To determine if TRAIL-induced apoptosis in human prostate tumor cells was suppressed by bcl-2, we compared the levels of apoptosis induced by recombinant human TRAIL in pairs of isogenic cell lines that do or do not express bcl-2. Three human prostate tumor cell lines (PC3, DU145 and LNCaP) and their bcl-2-expressing counterparts were tested for their susceptibility to TRAIL. Cells were exposed to TRAIL in the presence of cycloheximide which acted as a sensitizer. Apoptosis was induced rapidly in PC3 and DU145 neo-control transfected cells, whereas induction in LNCaP required 24 h. All three cell line variants expressing bcl-2 were resistant to the apoptotic effects of TRAIL. Caspase 3 and 8 activation was also detected in the neo control cells after treatment with TRAIL, demonstrating the rapid activation of the caspase cascade similar to that seen with other death receptors. Bcl-2 overexpression in these cells blocked activation of these caspases, suggesting that bcl-2 expression of human cancer cells may be a critical factor in the therapeutic efficacy of TRAIL.
AuthorsA Munshi, G Pappas, T Honda, T J McDonnell, A Younes, Y Li, R E Meyn
JournalOncogene (Oncogene) Vol. 20 Issue 29 Pg. 3757-65 (Jun 28 2001) ISSN: 0950-9232 [Print] England
PMID11439339 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antineoplastic Agents
  • Apoptosis Regulatory Proteins
  • BH3 Interacting Domain Death Agonist Protein
  • BID protein, human
  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • CFLAR protein, human
  • Carrier Proteins
  • Cytochrome c Group
  • Intracellular Signaling Peptides and Proteins
  • Ligands
  • Membrane Glycoproteins
  • Protein Synthesis Inhibitors
  • Proto-Oncogene Proteins c-bcl-2
  • Recombinant Proteins
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Tumor Necrosis Factor-alpha
  • Cycloheximide
  • Caspases
Topics
  • Antineoplastic Agents (metabolism, pharmacology)
  • Apoptosis
  • Apoptosis Regulatory Proteins
  • BH3 Interacting Domain Death Agonist Protein
  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • Carrier Proteins (biosynthesis, metabolism)
  • Caspases (metabolism)
  • Cycloheximide (pharmacology)
  • Cytochrome c Group (metabolism)
  • Enzyme Activation
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Ligands
  • Male
  • Membrane Glycoproteins (antagonists & inhibitors, metabolism, pharmacology)
  • Prostatic Neoplasms (drug therapy, metabolism)
  • Protein Synthesis Inhibitors (pharmacology)
  • Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
  • Recombinant Proteins (metabolism, pharmacology)
  • TNF-Related Apoptosis-Inducing Ligand
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha (antagonists & inhibitors, metabolism, pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: