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Large-scale preparation of the oligosaccharide phosphate fraction of Pichia holstii NRRL Y-2448 phosphomannan for use in the manufacture of PI-88.

Abstract
Mild acid-catalysed hydrolysis of the extracellular phosphomannan of the yeast Pichia holstii NRRL Y-2448 produces a high-molecular-weight phosphomannan core, a low-molecular-weight oligosaccharide phosphate fraction, and a neutral oligosaccharide fraction. A method was developed for the large-scale preparation of the oligosaccharide phosphate fraction, consisting predominantly of the pentasaccharide phosphate, 6-O-PO3H2-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 C 3)-alpha-D-Man-(1 --> 2)-D-Man, for use in the manufacture of the promising new anti-cancer agent, PI-88. Further insights were also gained into the structure of the phosphomannan by HPLC analysis of the time course of the hydrolysis reaction.
AuthorsV Ferro, K Fewings, M C Palermo, C Li
JournalCarbohydrate research (Carbohydr Res) Vol. 332 Issue 2 Pg. 183-9 (May 18 2001) ISSN: 0008-6215 [Print] Netherlands
PMID11434376 (Publication Type: Journal Article)
Chemical References
  • 6-phosphomannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-2-mannose
  • Antineoplastic Agents
  • Mannans
  • Oligosaccharides
  • phosphomannopentaose sulfate
  • phosphomannan
Topics
  • Antineoplastic Agents (chemical synthesis)
  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Industrial Microbiology (methods)
  • Mannans (chemistry, isolation & purification)
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Magnetic Resonance, Biomolecular
  • Oligosaccharides (chemical synthesis, chemistry, isolation & purification)
  • Pichia (chemistry)

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