Abstract |
Mild acid-catalysed hydrolysis of the extracellular phosphomannan of the yeast Pichia holstii NRRL Y-2448 produces a high-molecular-weight phosphomannan core, a low-molecular-weight oligosaccharide phosphate fraction, and a neutral oligosaccharide fraction. A method was developed for the large-scale preparation of the oligosaccharide phosphate fraction, consisting predominantly of the pentasaccharide phosphate, 6-O-PO3H2-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 C 3)-alpha-D-Man-(1 --> 2)-D-Man, for use in the manufacture of the promising new anti- cancer agent, PI-88. Further insights were also gained into the structure of the phosphomannan by HPLC analysis of the time course of the hydrolysis reaction.
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Authors | V Ferro, K Fewings, M C Palermo, C Li |
Journal | Carbohydrate research
(Carbohydr Res)
Vol. 332
Issue 2
Pg. 183-9
(May 18 2001)
ISSN: 0008-6215 [Print] Netherlands |
PMID | 11434376
(Publication Type: Journal Article)
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Chemical References |
- 6-phosphomannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-2-mannose
- Antineoplastic Agents
- Mannans
- Oligosaccharides
- phosphomannopentaose sulfate
- phosphomannan
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Topics |
- Antineoplastic Agents
(chemical synthesis)
- Carbohydrate Sequence
- Chromatography, High Pressure Liquid
- Hydrogen-Ion Concentration
- Hydrolysis
- Industrial Microbiology
(methods)
- Mannans
(chemistry, isolation & purification)
- Molecular Sequence Data
- Molecular Weight
- Nuclear Magnetic Resonance, Biomolecular
- Oligosaccharides
(chemical synthesis, chemistry, isolation & purification)
- Pichia
(chemistry)
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