Contribution of nitric oxide to the protective effects of ischemic preconditioning in ischemia-reperfused rat kidneys.

Abstract

We examined the contribution of nitric oxide (NO) to the effect of ischemic preconditioning (IP) on renal function and the hemodynamics in ischemia-reperfusion (I/R) mediated kidney injury. IP was performed by using 4 minutes of ischemia followed by a 30-minute reperfusion interval. I/R treatment consisted of a 30-minute ischemia and 60-minute reperfusion interval. We measured the glomerular filtration rate (GFR), the fractional excretion of sodium (FE(Na)), and the renal blood flow (RBF) in IP+I/R and I/R kidneys. Rats were pretreated with NaCl, N(G)-nitro-L-arginine methyl ester (L-NAME), or L-arginine. We found that IP significantly improved GFR and FE(Na) as compared with I/R treatment; however, this effect was completely abolished by L-NAME injection and enhanced by L-arginine treatment. L-NAME treatment significantly diminished RBF but did not alter nitrite/nitrate excretion. Furthermore, we found that IP alone does not lead to inducible NO synthase protein expression whereas I/R or IP+I/R treatment clearly did. Moreover, we observed an increased heme oxygenase-1 expression in IP+I/R kidneys as compared with I/R treated ones. Our results clearly showed that IP pretreatment protects kidneys from I/R mediated tissue injury and that these effects were partially mediated by NO.

• Authors: T Ogawa, A K Nussler, E Tuzuner, P Neuhaus, M Kaminishi, Y Mimura, H G Beger
• Journal: The Journal of laboratory and clinical medicine (J Lab Clin Med) Vol. 138 Issue 1 Pg. 50-8 (Jul 2001) ISSN: 0022-2143 [Print] United States
• PMID: 11433228 (Publication Type: Journal Article)

Chemical References

• Enzyme Inhibitors
• HSP70 Heat-Shock Proteins
• Nitric Oxide
• Arginine
• Sodium
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type II
• Nos2 protein, rat
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• Casp3 protein, rat
• Caspase 3
• Caspases
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Arginine (pharmacology)
• Blood Pressure (drug effects, physiology)
• Caspase 3
• Caspases (metabolism)
• Disease Models, Animal
• Enzyme Inhibitors (pharmacology)
• Glomerular Filtration Rate (drug effects, physiology)
• HSP70 Heat-Shock Proteins (metabolism)
• Heme Oxygenase (Decyclizing) (metabolism)
• Heme Oxygenase-1
• Ischemic Preconditioning
• Kidney (blood supply, enzymology)
• Male
• NG-Nitroarginine Methyl Ester (pharmacology)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase (metabolism)
• Nitric Oxide Synthase Type II
• Rats
• Rats, Wistar
• Reperfusion Injury (metabolism)
• Sodium (urine)