Chemopreventive effects of scordinin on diethylnitrosamine and phenobarbital-induced hepatocarcinogenesis in male F344 rats.

Abstract

Modifying effects of scordinin, a biological active component in garlic, on diethylnitrosamine (DEN)- and phenobarbital (PB)-induced hepatocarcinogenesis were examined in rats. Male F344 rats, 5 weeks old, were divided into 8 groups. After a week, groups 1 - 5 were given DEN (100 mg / kg body weight, i.p.) once a week for 3 weeks, whereas groups 6 - 8 received vehicle treatment. Group 2 was given 600 ppm scordinin-containing diet in the initiation phase. From 4 weeks after the start of experiment, groups 3 and 5 were fed scordinin, and groups 1 - 3 and 7 received drinking water containing 500 ppm PB. Group 6 was given scordinin diet alone throughout the experiment (24 weeks). The incidences of hepatocellular adenoma and carcinoma were significantly smaller in group 3 than those in group 1 (P < 0.005 and P < 0.05, respectively). The average numbers of liver neoplasms in groups 2 and 3 were significantly smaller than in group 1 (P < 0.01 and P < 0.0001, respectively). Glutathione S-transferase placental form-positive foci were also significantly decreased by scordinin treatment in the initiation or promotion phase. Scordinin significantly decreased the mean number of nucleolar organizer regions' protein (AgNORs) / nucleus in hepatocellular adenoma and carcinoma. AgNORs / nucleus in the non-lesional area was also significantly decreased by scordinin treatment during the promotion phase. These results suggest that scordinin is a promising chemopreventive agent for liver neoplasia.

• Authors: T Watanabe, S Sugie, K Okamoto, K M Rahman, J Ushida, H Mori
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 92 Issue 6 Pg. 603-9 (Jun 2001) ISSN: 0910-5050 [Print] Japan
• PMID: 11429047 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Carcinogens
• Plant Extracts
• scordinin
• Diethylnitrosamine
• Glutathione Transferase
• Phenobarbital

Topics

• Adenoma, Liver Cell (chemically induced, metabolism)
• Animals
• Anticarcinogenic Agents (pharmacology)
• Body Weight
• Carcinogens
• Carcinoma, Hepatocellular (chemically induced, metabolism)
• Cell Division
• Cell Nucleus (metabolism)
• Diethylnitrosamine
• Dose-Response Relationship, Drug
• Glutathione Transferase (metabolism)
• Liver (drug effects, pathology)
• Liver Neoplasms (chemically induced, metabolism)
• Male
• Models, Chemical
• Neoplasms (prevention & control)
• Nucleolus Organizer Region (metabolism)
• Organ Size
• Phenobarbital
• Plant Extracts (pharmacology)
• Rats
• Rats, Inbred F344
• Time Factors