Characterization of 5' flanking region of alpha isoform of rat Ca2+/calmodulin-dependent protein kinase II gene and neuronal cell type specific promoter activity.

Abstract

The 5' flanking region of the alpha isoform of the rat Ca2+/calmodulin-dependent protein kinase II (alpha CaM kinase II) gene was isolated in 2.3 kbp of genomic sequence. Functional analysis of alpha CaM kinase II promoter deletion mutants fused to a reporter gene in neuroblastoma, including N18TG2, NG108-15, and CAD cells revealed strong transcriptional activity localized 100-145 bp, and a potent silencer 199-275 bp upstream of the transcription start site. The promoter is inactive in non-neuronal cells including BALB/c 3T3, Chinese hamster ovary, HT1080, and C6 glioma cells. These results indicated that the alpha CaM kinase II gene is transcribed from a tissue-specific promoter which is under intense negative control.

• Authors: K Mima, S Deguchi, T Yamauchi
• Journal: Neuroscience letters (Neurosci Lett) Vol. 307 Issue 2 Pg. 117-21 (Jul 13 2001) ISSN: 0304-3940 [Print] Ireland
• PMID: 11427314 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Isoforms
• Luciferases
• CAMK2A protein, human
• Calcium-Calmodulin-Dependent Protein Kinase Type 2
• Calcium-Calmodulin-Dependent Protein Kinases
• Camk2a protein, mouse
• Camk2a protein, rat

Topics

• Animals
• Base Sequence (physiology)
• Binding Sites (genetics)
• Brain (cytology, enzymology)
• Brain Chemistry (genetics)
• Calcium-Calmodulin-Dependent Protein Kinase Type 2
• Calcium-Calmodulin-Dependent Protein Kinases (genetics)
• Gene Silencing (physiology)
• Genes (genetics)
• Genes, Reporter (genetics)
• Humans
• Luciferases (genetics)
• Mice
• Molecular Sequence Data
• Neurons (cytology, enzymology)
• Promoter Regions, Genetic (genetics)
• Protein Isoforms (genetics)
• Transcription, Genetic (genetics)
• Transfection
• Tumor Cells, Cultured (metabolism)