Nucleocytoplasmic shuttling of endocytic proteins.

Abstract	Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and alpha-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.
Authors	M Vecchi, S Polo, V Poupon, J W van de Loo, A Benmerah, P P Di Fiore
Journal	The Journal of cell biology (J Cell Biol) Vol. 153 Issue 7 Pg. 1511-7 (Jun 25 2001) ISSN: 0021-9525 [Print] United States
PMID	11425879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Adaptor Protein Complex alpha Subunits Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Calcium-Binding Proteins Carrier Proteins DNA-Binding Proteins EPS15 protein, human Fungal Proteins GAL4 protein, S cerevisiae Intracellular Signaling Peptides and Proteins Membrane Proteins Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins Neuropeptides PICALM protein, human Phosphoproteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Vesicular Transport Proteins epsin
Topics	Active Transport, Cell Nucleus (physiology) Adaptor Protein Complex alpha Subunits Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Animals Calcium-Binding Proteins (metabolism) Carrier Proteins (metabolism) Cell Line Cell Nucleus (metabolism) Chlorocebus aethiops Cytoplasm (metabolism) DNA-Binding Proteins Endocytosis (physiology) Fungal Proteins (genetics, metabolism) HeLa Cells Humans Intracellular Signaling Peptides and Proteins Membrane Proteins (metabolism) Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins (metabolism) Neuropeptides (metabolism) Phosphoproteins (metabolism) Protein Transport (physiology) Recombinant Fusion Proteins (genetics, metabolism) Saccharomyces cerevisiae Proteins Signal Transduction (physiology) Transcription Factors (genetics, metabolism) Transcription, Genetic (physiology) Transcriptional Activation (physiology) Vesicular Transport Proteins

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