Use of intravaginal microbicides to prevent acquisition of Trichomonas vaginalis infection in Lactobacillus-pretreated, estrogenized young mice.

Abstract

D2A21, a novel peptide antibiotic has in vitro activity against a wide spectrum of sexually transmitted diseases (STD). In this study we tested the hypothesis that intravaginal D2A21 would interfere with acquisition of Trichomonas vaginalis infection in a modified mouse model. T. vaginalis infections of estrogenized young mice pretreated with Lactobacillus vaginalis or Lactobacillus rhamnosus were more frequent and persistent than those in mice pre-treated with Lactobacillus gasseri or Lactobacillus acidophilus. One hundred percent T. vaginalis infection was achieved for 2-4 days post-challenge when intravaginal L. rhamnosus pre-treatments were given to estrogenized mice 48 hr prior to a single T. vaginalis challenge. Estrogenized mice pre-treated with L. rhamnosus were pre-medicated with intravaginal placebo gel, 0.5% or 2% D2A21 gel, or 500 microg/mL metronidazole gel prior to T. vaginalis challenge. Both 2% D2A21 and metronidazole gels were significantly more efficacious (10% or none infected) than placebo gel (53% infected) in preventing vaginal T. vaginalis infections in mice.

• Authors: W B Lushbaugh, A C Blossom, P H Shah, A K Banga, J M Jaynes, J D Cleary, R W Finley
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) 2000 Nov-Dec Vol. 63 Issue 5-6 Pg. 284-9 ISSN: 0002-9637 [Print] United States
• PMID: 11421379 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anti-Bacterial Agents
• Antimicrobial Cationic Peptides
• D2A21
• Estrogens
• Gels
• Peptides
• Metronidazole

Topics

• Administration, Intravaginal
• Animals
• Anti-Bacterial Agents (administration & dosage, pharmacology)
• Antimicrobial Cationic Peptides
• Disease Models, Animal
• Estrogens
• Female
• Gels
• Lactobacillus
• Metronidazole (administration & dosage, pharmacology)
• Mice
• Mice, Inbred BALB C
• Peptides
• Trichomonas Vaginitis (prevention & control)
• Trichomonas vaginalis (drug effects)