Abstract | BACKGROUND:
Anaplastic thyroid carcinoma is an aggressive solid tumor that fails to adequately respond to any known chemotherapeutic regimen. The development of effective chemotherapy agents would provide the best chance for long-term survival of patients. MATERIALS AND METHODS: RESULTS: All the tumor cell lines exhibited marked sensitivity against BS-RNase in comparison to HFF and RPE cells. The greatest growth inhibition was seen in the 8505C line, while IC50 values for papillary (B-CPAP) and poorly-differentiated thyroid carcinoma cells were about 6-fold higher. The cytotoxic action of BS-RNase was associated with induction of apoptosis. Expressions of Fas and Fas-ligand were not influenced by BS-RNase completely, while the down-regulation of Bcl-2 in treated cells was observed. In vivo treatment induced significant tumor regression after the course of 20 consecutive days. No apparent toxic effects of BS-RNase toward non-malignant cells were observed during the in vivo treatment. After cessation of therapy (day 20) tumor volume continued to decrease and the tumor was no longer detectable after 30 days of treatment induction in all animals. CONCLUSION:
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Authors | R Kotchetkov, J Cinatl, A A Krivtchik, J U Vogel, J Matousek, P Pouckova, B Kornhuber, D Schwabe, J Cinatl Jr |
Journal | Anticancer research
(Anticancer Res)
2001 Mar-Apr
Vol. 21
Issue 2A
Pg. 1035-42
ISSN: 0250-7005 [Print] Greece |
PMID | 11396137
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- FASLG protein, human
- Fas Ligand Protein
- Fasl protein, mouse
- Membrane Glycoproteins
- Proto-Oncogene Proteins c-bcl-2
- fas Receptor
- Endoribonucleases
- ribonuclease SPL
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Apoptosis
- Cattle
- Endoribonucleases
(pharmacology, therapeutic use)
- Fas Ligand Protein
- Female
- Humans
- Membrane Glycoproteins
(biosynthesis)
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Neoplasms, Experimental
- Proto-Oncogene Proteins c-bcl-2
(biosynthesis)
- Thyroid Neoplasms
(drug therapy, pathology)
- Tumor Cells, Cultured
- fas Receptor
(biosynthesis)
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