Abstract |
Immunoglobulin A ( IgA) is important in protective immunity against infection by Vibrio cholerae. In this study, the immune response to and protective role of a 31-kDa antigen of V. cholerae O139, reacting with IgA antibodies present in the sera of cholera patients and common to V. cholerae strains O139 and O1 was evaluated in BALB/c mice. From the various antigens of V. cholerae O139 and V. cholerae O1 which reacted with IgA antibodies in sera of a cholera patient, a 31-kDa common antigen was selected and purified by DEAE- Sepharose CL 6B column chromatography. Oral administration of live V. cholerae O139 in BALB/c mice elicited an IgA response to the 31-kDa antigen in serum and intestinal fluid, and a proliferation of the splenic lymphocytes on stimulation with the same antigen. The cytokine profile of these splenic lymphocytes revealed a shift from a mixed Th1 and Th2 response--interleukin-10 (IL-10) and interferon-gamma--in the first week after infection to a Th2 type of response--IL-10--in the third week. In passive protection studies, hyperimmune serum to the 31-kDa antigen was able to protect infant mice against challenge with O139 and O1 strains. These results demonstrate the ability of the 31-kDa antigen of V. cholerae O139 to induce humoral and cellular immune responses in mice, and its immunoprotective nature.
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Authors | Subodh Kumar, Pallab Ray, Harminder Singh, Nirmal Kumar Ganguly |
Journal | Journal of medical microbiology
(J Med Microbiol)
Vol. 50
Issue 6
Pg. 489-498
(Jun 2001)
ISSN: 0022-2615 [Print] England |
PMID | 11393286
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
- Cytokines
- Immunoglobulin A
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Topics |
- Animals
- Animals, Newborn
- Antibodies, Bacterial
(biosynthesis, blood, immunology)
- Antigens, Bacterial
(analysis, immunology, isolation & purification)
- Cholera
(blood, immunology)
- Chromatography, DEAE-Cellulose
- Cytokines
(biosynthesis)
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Immunity, Cellular
- Immunoblotting
- Immunoglobulin A
(biosynthesis, blood, immunology)
- Lymphocyte Activation
- Lymphocytes
(immunology)
- Mice
- Mice, Inbred BALB C
- Spleen
(cytology, immunology)
- Vibrio cholerae
(immunology)
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