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Immunogenicity and protective role of an IgA reactive 31-kDa antigen of Vibrio cholerae O139.

Abstract
Immunoglobulin A (IgA) is important in protective immunity against infection by Vibrio cholerae. In this study, the immune response to and protective role of a 31-kDa antigen of V. cholerae O139, reacting with IgA antibodies present in the sera of cholera patients and common to V. cholerae strains O139 and O1 was evaluated in BALB/c mice. From the various antigens of V. cholerae O139 and V. cholerae O1 which reacted with IgA antibodies in sera of a cholera patient, a 31-kDa common antigen was selected and purified by DEAE-Sepharose CL 6B column chromatography. Oral administration of live V. cholerae O139 in BALB/c mice elicited an IgA response to the 31-kDa antigen in serum and intestinal fluid, and a proliferation of the splenic lymphocytes on stimulation with the same antigen. The cytokine profile of these splenic lymphocytes revealed a shift from a mixed Th1 and Th2 response--interleukin-10 (IL-10) and interferon-gamma--in the first week after infection to a Th2 type of response--IL-10--in the third week. In passive protection studies, hyperimmune serum to the 31-kDa antigen was able to protect infant mice against challenge with O139 and O1 strains. These results demonstrate the ability of the 31-kDa antigen of V. cholerae O139 to induce humoral and cellular immune responses in mice, and its immunoprotective nature.
AuthorsSubodh Kumar, Pallab Ray, Harminder Singh, Nirmal Kumar Ganguly
JournalJournal of medical microbiology (J Med Microbiol) Vol. 50 Issue 6 Pg. 489-498 (Jun 2001) ISSN: 0022-2615 [Print] England
PMID11393286 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Cytokines
  • Immunoglobulin A
Topics
  • Animals
  • Animals, Newborn
  • Antibodies, Bacterial (biosynthesis, blood, immunology)
  • Antigens, Bacterial (analysis, immunology, isolation & purification)
  • Cholera (blood, immunology)
  • Chromatography, DEAE-Cellulose
  • Cytokines (biosynthesis)
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Immunity, Cellular
  • Immunoblotting
  • Immunoglobulin A (biosynthesis, blood, immunology)
  • Lymphocyte Activation
  • Lymphocytes (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Spleen (cytology, immunology)
  • Vibrio cholerae (immunology)

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