Abstract |
We assessed the immunogenicities and efficacies of two highly attenuated vaccinia virus-derived NYVAC vaccine candidates encoding the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) env gene or both the env and gag genes in prime-boost pilot regimens in combination with naked DNA expressing the HTLV-1 envelope. Three inoculations of NYVAC HTLV-1 env at 0, 1, and 3 months followed by a single inoculation of DNA env at 9 months protected against intravenous challenge with HTLV-1-infected cells in one of three immunized squirrel monkeys. Furthermore, humoral and cell-mediated immune responses against HTLV-1 Env could be detected in this protected animal. However, priming the animal with a single dose of env DNA, followed by immunization with the NYVAC HTLV-1 gag and env vaccine at 6, 7, and 8 months, protected all three animals against challenge with HTLV-1-infected cells. With this protocol, antibodies against HTLV-1 Env and cell-mediated responses against Env and Gag could also be detected in the protected animals. Although the relative superiority of a DNA prime-NYVAC boost regimen over addition of the Gag component as an immunogen cannot be assessed directly, our findings nevertheless show that an HTLV-1 vaccine approach is feasible and deserves further study.
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Authors | M Kazanji, J Tartaglia, G Franchini, B de Thoisy, A Talarmin, H Contamin, A Gessain, G de Thé |
Journal | Journal of virology
(J Virol)
Vol. 75
Issue 13
Pg. 5939-48
(Jul 2001)
ISSN: 0022-538X [Print] United States |
PMID | 11390595
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Gene Products, env
- Gene Products, gag
- Vaccines, DNA
- Vaccines, Synthetic
- Viral Vaccines
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Topics |
- Animals
- Gene Products, env
(immunology)
- Gene Products, gag
(immunology)
- Human T-lymphotropic virus 1
(immunology)
- Immunization
- Male
- Saimiri
- Vaccines, DNA
(immunology)
- Vaccines, Synthetic
(immunology)
- Viral Vaccines
(immunology)
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