Imaging infection with LeuTech.

Abstract

LeuTech is a 99Tcm labelled, anti-CD15, IgM, murine monoclonal antibody shown to have high affinity (Kd = 10(-11) M) for CD15 receptors (5.1 x 10(5)/cell) expressed on human neutrophils. LeuTech was injected directly, intravenously, and its efficacy in imaging infection in 46 consecutive patients was determined. Human anti-mouse antibody (HAMA) response was examined in 30 normal volunteers using a standard LeuTech dose reconstituted with decayed 99Tcm solution. There were 38 true positive, six true negative, and two false negative scans. Of the 38 positive images, 33 (92%) were positive within 10 min after injection of LeuTech. LeuTech accuracy in this group of patients was 96%, sensitivity 95%, specificity 100%, positive predictive value (PPV) 100%, and negative predictive value (NPV) 75%. No elevation of the HAMA titre was observed in any of the 30 normal volunteers and no adverse reaction was noted in any patient. LeuTech is a highly promising agent for rapid imaging of infectious foci.

• Authors: M L Thakur, C S Marcus, S L Kipper, R Ahdoot, L Diggles, H L Pham, C Minami, Rhodes, M C Patel, T Smith
• Journal: Nuclear medicine communications (Nucl Med Commun) Vol. 22 Issue 5 Pg. 513-9 (May 2001) ISSN: 0143-3636 [Print] England
• PMID: 11388572 (Publication Type: Journal Article, Multicenter Study)

Chemical References

• Antibodies, Monoclonal
• Lewis X Antigen
• Radiopharmaceuticals
• techneticum TC-99M fanolesomab

Topics

• Adult
• Animals
• Antibodies, Monoclonal
• Appendicitis (diagnostic imaging)
• Bone Diseases (diagnostic imaging)
• False Negative Reactions
• Female
• Humans
• Infections (diagnostic imaging)
• Lewis X Antigen (immunology)
• Lung Diseases (diagnostic imaging)
• Male
• Mice
• Middle Aged
• Predictive Value of Tests
• Radiopharmaceuticals
• Reference Values
• Reproducibility of Results
• Sensitivity and Specificity
• Tomography, Emission-Computed, Single-Photon