[Cloning of rat beta-defensin rBD-1 cDNA].

Abstract

This is a study aimed at the molecular mechanisms of beta-defensins gene expression and it's gene transfer experiments for preventing mucosal infection. A rat rBD-1 cDNA was cloned. The total RNA was isolated from rat kidney. The cDNA fragment was amplified by RT-PCR with specific primers. The purified RT-PCR product was cloned in pGEM-T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD-1 and mBD-1 demonstrated that rat beta-defensin rBD-1 gene was cloned successfully.

• Authors: N Huang, B Tang, Q Wu, X Chen, X Pan, B Wang
• Journal: Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao (Hua Xi Yi Ke Da Xue Xue Bao) Vol. 30 Issue 4 Pg. 357-9, 362 (Dec 1999) ISSN: 0257-7712 [Print] China
• PMID: 11387938 (Publication Type: English Abstract, Journal Article)

Chemical References

• Anti-Infective Agents
• DNA, Complementary
• Recombinant Proteins
• beta-Defensins

Topics

• Amino Acid Sequence
• Animals
• Anti-Infective Agents
• Base Sequence
• Cloning, Molecular
• DNA, Complementary (genetics)
• Gene Expression Regulation
• Kidney (metabolism)
• Molecular Sequence Data
• Rats
• Rats, Wistar
• Recombinant Proteins (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• beta-Defensins (biosynthesis, genetics)