Specific interaction between the ribosome recycling factor and the elongation factor G from Mycobacterium tuberculosis mediates peptidyl-tRNA release and ribosome recycling in Escherichia coli.

Abstract	Once the translating ribosomes reach a termination codon, the nascent polypeptide chain is released in a factor-dependent manner. However, the P-site-bound deacylated tRNA and the ribosomes themselves remain bound to the mRNA (post-termination complex). The ribosome recycling factor (RRF) plays a vital role in dissociating this complex. Here we show that the Mycobacterium tuberculosis RRF (MtuRRF) fails to rescue Escherichia coli LJ14, a strain temperature-sensitive for RRF (frr(ts)). More interestingly, co-expression of M.tuberculosis elongation factor G (MtuEFG) with MtuRRF rescues the frr(ts) strain of E.coli. The simultaneous expression of MtuEFG is also needed to cause an enhanced release of peptidyl-tRNAs in E.coli by MtuRRF. These observations provide the first genetic evidence for a functional interaction between RRF and EFG. Both the in vivo and in vitro analyses suggest that RRF does not distinguish between the translating and terminating ribosomes for their dissociation from mRNA. In addition, complementation of E.coli PEM100 (fusA(ts)) with MtuEFG suggests that the mechanism of RRF function is independent of the translocation activity of EFG.
Authors	A R Rao, U Varshney
Journal	The EMBO journal (EMBO J) Vol. 20 Issue 11 Pg. 2977-86 (Jun 01 2001) ISSN: 0261-4189 [Print] England
PMID	11387230 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Peptide Elongation Factor G Proteins RNA, Transfer, Amino Acyl Recombinant Proteins Ribosomal Proteins ribosome releasing factor tRNA, peptidyl- RNA, Transfer
Topics	Amino Acid Sequence Cloning, Molecular Escherichia coli (genetics, growth & development, metabolism) Kinetics Molecular Sequence Data Mycobacterium tuberculosis (genetics, metabolism) Peptide Elongation Factor G (metabolism) Plasmids Proteins (chemistry, genetics, metabolism) RNA, Transfer (metabolism) RNA, Transfer, Amino Acyl (metabolism) Recombinant Proteins (metabolism) Ribosomal Proteins Ribosomes (metabolism) Sequence Alignment Sequence Homology, Amino Acid Temperature

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