Abstract |
There are three main routes for the utilization of fructose by Escherichia coli. One (Route A) predominates in the growth of wild-type strains. It involves the functioning of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) and a fructose operon, mapping at min. 48.7, containing genes for a membrane-spanning protein (fruA), a 1-phosphofructose kinase (fruK) and a diphosphoryl transfer protein (fruB), under negative regulation by a fruR gene mapping at min. 1.9. A second route (Route B) also involves the PTS and membrane-spanning proteins that recognize a variety of sugars possessing the 3,4,5-D-arabino-hexoseconfiguration but with primary specificity for mannose(manXYZ), mannitol (mtlA) and glucitol (gutA) and which, if over-produced, can transport also fructose. A third route (Route C), functioning in mutants devoid of Routes A and B, does not involve the PTS: fructose diffuses into the cell via an isoform (PtsG-F) of the major glucose permease of the PTS and is then phosphorylated by ATP and a manno(fructo)kinase (Mak+) specified by a normally cryptic 1032 bp ORF (yajF) of hitherto unknown function (Mak-o), mapping at min. 8.8 and corresponding to a peptide of 344 amino acids. Conversion of the Mak-o to the Mak+ phenotypeinvolves an A24D mutation in a putative regulatory region.
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Authors | H L Kornberg |
Journal | Journal of molecular microbiology and biotechnology
(J Mol Microbiol Biotechnol)
Vol. 3
Issue 3
Pg. 355-9
(Jul 2001)
ISSN: 1464-1801 [Print] Switzerland |
PMID | 11361065
(Publication Type: Journal Article, Review)
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Chemical References |
- Bacterial Proteins
- FruA protein, Myxococcus xanthus
- Transcription Factors
- Fructose
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Phosphofructokinase-1
- 1-phosphofructokinase
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Topics |
- Bacterial Proteins
- Escherichia coli
(enzymology, genetics, growth & development)
- Fructose
(metabolism)
- Operon
- Phosphoenolpyruvate Sugar Phosphotransferase System
(genetics, metabolism)
- Phosphofructokinase-1
(genetics)
- Transcription Factors
(genetics)
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